Hypermethylation of Long Non-Coding RNA Genes Group in the Breast Cancer Development and Progression

被引：4
作者：
Selezneva, A. D. [1 ]
Filippova, E. A. [2 ]
Lukina, S. S. [2 ]
Pronina, I., V [2 ]
Ivanova, N. A. [2 ]
Kazubskaya, T. P. [3 ]
Burdennyy, A. M. [2 ]
Braga, E. A. [2 ]
Loginov, V., I [2 ]
机构：
[1] Moscow MV Lomonosov State Univ, Fac Biol, Moscow, Russia
[2] Res Inst Gen Pathol & Pathophysiol, Moscow, Russia
[3] Minist Hlth Russian Federat, NN Blokhin Natl Med Res Ctr Oncol, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2022年 / 173卷 / 06期
基金：
俄罗斯科学基金会;
关键词：
genes; long non-coding RNAs; methylation; breast cancer;
D O I：
10.1007/s10517-022-05627-8
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We analyzed changes in the level of methylation of CpG islands in four long non-coding RNA (lncRNA) genes MEG3, ZNF667-AS1, GAS5, and SEMA3B-AS1 as promising markers of breast cancer. Methylation analysis was performed by quantitative methylation-specific PCR on a set of 38 paired (tumor/normal) breast cancer samples. Significantly (p<0.001) increased methylation was shown for three of the four lncRNA genes: MEG3, ZNF667-AS1, and SEMA3B-AS1. We found significant correlations of the methylation level of all the studied lncRNA genes with the stage of cancer and with lymphogenic metastasis, and for MEG3 and ZNF667-AS1 also with the tumor size. Methylation of ZNF667-AS1, and SEMA3B-AS1 genes in breast cancer was detected for the first time. Based on these findings, new potential markers for the diagnosis and prognosis of breast cancer can be proposed.
引用
收藏
页码：765 / 769
页数：5
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