Physicochemical properties and in vitro antioxidant activities of polysaccharide from Artocarpus heterophyllus Lam. pulp

被引:86
作者
Zhu, Kexue [1 ]
Zhang, Yanjun [1 ]
Nie, Shaoping [2 ]
Xu, Fei [1 ]
He, Shuzhen [1 ]
Gong, Deming [3 ]
Wu, Gang [1 ]
Tan, Lehe [1 ]
机构
[1] Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning 571533, Hainan, Peoples R China
[2] Nanchang Univ, State Key Lab Food Sci & Technol, 235 Nanjing East Rd, Nanchang 330047, Peoples R China
[3] Univ Auckland, Sch Biol Sci, Private Bag 92019, Auckland, New Zealand
关键词
Artocarpus heterophyllus Lam; Polysaccharide; Physicochemical properties; Antioxidant activity; MOLECULAR-WEIGHT POLYSACCHARIDES; WATER-SOLUBLE POLYSACCHARIDES; ANION-EXCHANGE CHROMATOGRAPHY; CYCLOCARYA-PALIURUS; STRUCTURAL-CHARACTERIZATION; GANODERMA-ATRUM; FT-IR; EXTRACTION; PURIFICATION; FRUITS;
D O I
10.1016/j.carbpol.2016.08.074
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A water-soluble polysaccharide from Artocarpus heterophyllus Lam. (jackfruit) pulp (JFP-Ps) was purified and its physicochemical properties were investigated. The in vitro antioxidant activities of JFP-Ps was evaluated by measuring DPPH center dot and (OH)-O-center dot radicals scavenging activities, as well as reducing power. The results showed that JFP-Ps contained 79.12% of total sugar, 5.83% of protein, 15.65% of uronic acid, and 15 kinds of amino acids with high levels of Asp, Glu, Val, Leu and Lys. JFP-Ps was mainly composed of Rha, Ara, Gal, Glc, Xyl and GalA, with an average molecular weight of 1668 kDa. FT-IR results showed the bands at the range of 1200-850 cm(-1) suggested the presence of carbohydrates in JFP-Ps. The results of antioxidant activities showed that JFP-Ps exhibited strong DPPH center dot and (OH)-O-center dot radical scavenging activities, with a relatively lower reducing power, suggesting that JFP-Ps can be exploited as effective natural antioxidant applications in medical and food industries. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:354 / 361
页数:8
相关论文
共 41 条
[1]   Phytochemistry, nutritional and pharmacological properties of Artocarpus heterophyllus Lam (jackfruit): A review [J].
Baliga, Manjeshwar Shrinath ;
Shivashankara, Arnadi Ramachandrayya ;
Haniadka, Raghavendra ;
Dsouza, Jerome ;
Bhat, Harshith P. .
FOOD RESEARCH INTERNATIONAL, 2011, 44 (07) :1800-1811
[2]   NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS [J].
BLUMENKR.N ;
ASBOEHAN.G .
ANALYTICAL BIOCHEMISTRY, 1973, 54 (02) :484-489
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   Optimization extraction, characterization and antioxidant activities of pectic polysaccharide from tangerine peels [J].
Chen, Ruizhan ;
Jin, Chenguang ;
Tong, Zhigang ;
Lu, Juan ;
Tan, Li ;
Tian, Li ;
Chang, Qingquan .
CARBOHYDRATE POLYMERS, 2016, 136 :187-197
[5]   In Vitro Antioxidant Activities of Low-Molecular-Weight Polysaccharides with Various Functional Groups [J].
Chen, Szu Kai ;
Tsai, Min Lang ;
Huang, Jin Ru ;
Chen, Rong Huei .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (07) :2699-2704
[6]   Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum [J].
Chen, Yi ;
Xie, Ming-Yong ;
Nie, Shao-Ping ;
Li, Chang ;
Wang, Yuan-Xing .
FOOD CHEMISTRY, 2008, 107 (01) :231-241
[7]   Multivariate analysis of uronic acid and neutral sugars in whole pectic samples by FT-IR spectroscopy [J].
Coimbra, MA ;
Barros, A ;
Barros, M ;
Rutledge, DN ;
Delgadillo, I .
CARBOHYDRATE POLYMERS, 1998, 37 (03) :241-248
[8]   Purification, characterization and antioxidant activities of polysaccharides from thinned-young apple [J].
Dou, Jiao ;
Meng, Yonghong ;
Liu, Lei ;
Li, Jie ;
Ren, Daoyuan ;
Guo, Yurong .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2015, 72 :31-40
[9]   A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].
DUBOIS, M ;
GILLES, K ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
NATURE, 1951, 168 (4265) :167-167
[10]   Anti-inflammatory effects of phenolic compounds isolated from the fruits of Artocarpus heterophyllus [J].
Fang, Song-Chwan ;
Hsu, Chin-Lin ;
Yen, Gow-Chin .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (12) :4463-4468