Multicolor two-photon tissue imaging by wavelength mixing

被引：1

作者：

Mahou, Pierre ^{[1
,2
]}

Zimmerley, Maxwell ^{[1
,2
]}

Loulier, Karine ^{[3
,4
]}

Matho, Katherine S. ^{[3
,4
]}

Labroille, Guillaume ^{[1
,2
]}

Morin, Xavier ^{[5
,6
]}

Supatto, Willy ^{[1
,2
]}

Livet, Jean ^{[3
]}

Debarre, Delphine ^{[1
,2
]}

Beaurepaire, Emmanuel ^{[1
,2
]}

机构：

[1] Ecole Polytech, CNRS, Lab Opt & Biosci, UMR 7645, F-91128 Palaiseau, France

[2] INSERM, U696, Palaiseau, France

[3] UPMC Univ Paris 6, Inst Vis, INSERM, U968,UMR S968, Paris, France

[4] CNRS, UMR 7210, Paris, France

[5] Ecole Normale Super, CNRS, Inst Biol, UMR 8197, Paris, France

[6] INSERM, U1024, Paris, France

来源：

NATURE METHODS | 2012年 / 9卷 / 08期

关键词：

3RD-HARMONIC GENERATION MICROSCOPY; FLUORESCENT PROTEINS; MULTIPHOTON MICROSCOPY; NERVOUS-SYSTEM; EXCITATION; DROSOPHILA; EMBRYOS; CELLS; DEEP;

D O I：

10.1038/NMETH.2098

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We achieve simultaneous two-photon excitation of three chromophores with distinct absorption spectra using synchronized pulses from a femtosecond laser and an optical parametric oscillator. The two beams generate separate multiphoton processes, and their spatiotemporal overlap provides an additional two-photon excitation route, with submicrometer overlay of the color channels. We report volume and live multicolor imaging of 'Brainbow'-labeled tissues as well as simultaneous three-color fluorescence and third-harmonic imaging of fly embryos.

引用

页码：815 / +

页数：7

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