Multicolor two-photon tissue imaging by wavelength mixing

被引：1
作者：
Mahou, Pierre [1 ,2 ]
Zimmerley, Maxwell [1 ,2 ]
Loulier, Karine [3 ,4 ]
Matho, Katherine S. [3 ,4 ]
Labroille, Guillaume [1 ,2 ]
Morin, Xavier [5 ,6 ]
Supatto, Willy [1 ,2 ]
Livet, Jean [3 ]
Debarre, Delphine [1 ,2 ]
Beaurepaire, Emmanuel [1 ,2 ]
机构：
[1] Ecole Polytech, CNRS, Lab Opt & Biosci, UMR 7645, F-91128 Palaiseau, France
[2] INSERM, U696, Palaiseau, France
[3] UPMC Univ Paris 6, Inst Vis, INSERM, U968,UMR S968, Paris, France
[4] CNRS, UMR 7210, Paris, France
[5] Ecole Normale Super, CNRS, Inst Biol, UMR 8197, Paris, France
[6] INSERM, U1024, Paris, France
来源：
NATURE METHODS | 2012年 / 9卷 / 08期
关键词：
3RD-HARMONIC GENERATION MICROSCOPY; FLUORESCENT PROTEINS; MULTIPHOTON MICROSCOPY; NERVOUS-SYSTEM; EXCITATION; DROSOPHILA; EMBRYOS; CELLS; DEEP;
D O I：
10.1038/NMETH.2098
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We achieve simultaneous two-photon excitation of three chromophores with distinct absorption spectra using synchronized pulses from a femtosecond laser and an optical parametric oscillator. The two beams generate separate multiphoton processes, and their spatiotemporal overlap provides an additional two-photon excitation route, with submicrometer overlay of the color channels. We report volume and live multicolor imaging of 'Brainbow'-labeled tissues as well as simultaneous three-color fluorescence and third-harmonic imaging of fly embryos.
引用
收藏
页码：815 / +
页数：7
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