Multicolor two-photon tissue imaging by wavelength mixing

被引：1

作者：

Mahou, Pierre ^{[1
,2
]}

Zimmerley, Maxwell ^{[1
,2
]}

Loulier, Karine ^{[3
,4
]}

Matho, Katherine S. ^{[3
,4
]}

Labroille, Guillaume ^{[1
,2
]}

Morin, Xavier ^{[5
,6
]}

Supatto, Willy ^{[1
,2
]}

Livet, Jean ^{[3
]}

Debarre, Delphine ^{[1
,2
]}

Beaurepaire, Emmanuel ^{[1
,2
]}

机构：

[1] Ecole Polytech, CNRS, Lab Opt & Biosci, UMR 7645, F-91128 Palaiseau, France

[2] INSERM, U696, Palaiseau, France

[3] UPMC Univ Paris 6, Inst Vis, INSERM, U968,UMR S968, Paris, France

[4] CNRS, UMR 7210, Paris, France

[5] Ecole Normale Super, CNRS, Inst Biol, UMR 8197, Paris, France

[6] INSERM, U1024, Paris, France

来源：

NATURE METHODS | 2012年 / 9卷 / 08期

关键词：

3RD-HARMONIC GENERATION MICROSCOPY; FLUORESCENT PROTEINS; MULTIPHOTON MICROSCOPY; NERVOUS-SYSTEM; EXCITATION; DROSOPHILA; EMBRYOS; CELLS; DEEP;

D O I：

10.1038/NMETH.2098

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We achieve simultaneous two-photon excitation of three chromophores with distinct absorption spectra using synchronized pulses from a femtosecond laser and an optical parametric oscillator. The two beams generate separate multiphoton processes, and their spatiotemporal overlap provides an additional two-photon excitation route, with submicrometer overlay of the color channels. We report volume and live multicolor imaging of 'Brainbow'-labeled tissues as well as simultaneous three-color fluorescence and third-harmonic imaging of fly embryos.

引用

页码：815 / +

页数：7

共 50 条

[1] Multicolor two-photon tissue imaging by wavelength mixing
Mahou P.
Zimmerley M.
Loulier K.
Matho K.S.
Labroille G.
Morin X.
Supatto W.
Livet J.
Débarre D.
Beaurepaire E.
Nature Methods, 2012, 9 (8) : 815 - 818
[2] Multicolor two-photon imaging of endogenous fluorophores in living tissues by wavelength mixing
Chiara Stringari
Lamiae Abdeladim
Guy Malkinson
Pierre Mahou
Xavier Solinas
Isabelle Lamarre
Sébastien Brizion
Jean-Baptiste Galey
Willy Supatto
Renaud Legouis
Ana-Maria Pena
Emmanuel Beaurepaire
Scientific Reports, 7
[3] Multicolor two-photon imaging of endogenous fluorophores in living tissues by wavelength mixing
Stringari, Chiara
Abdeladim, Lamiae
Malkinson, Guy
Mahou, Pierre
Solinas, Xavier
Lamarre, Isabelle
Brizion, Sebastien
Galey, Jean-Baptiste
Supatto, Willy
Legouis, Renaud
Pena, Ana-Maria
Beaurepaire, Emmanuel
SCIENTIFIC REPORTS, 2017, 7
[4] An Efficient Multicolor Two-Photon Imaging of Endogenous Fluorophores in Living Tissues by Wavelength Mixing
Stringari, Chiara
Abdeladim, Lamiae
Malkinson, Guy
Supatto, Willy
Brizion, Sebastien
Galey, Jean-Baptiste
Pena, Ana-Maria
Legouis, Renaud
Beaurepaire, Emmanuel
BIOPHYSICAL JOURNAL, 2017, 112 (03) : 186A - 186A
[5] Two-photon absorption imaging of tissue.
Warren, WS
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2004, 228 : U824 - U824
[6] Multicolor excitation two-photon microscopy: in vivo imaging of cells and tissues
Li, Dong
Zheng, Wei
Qu, Jianan Y.
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES X, 2010, 7569
[7] Two-photon imaging in intact lymphoid tissue
Wei, SH
Miller, MJ
Cahalan, MD
Parker, I
LYMPHOCYTE ACTIVATION AND IMMUNE REGULATION IX: HOMEOSTASIS AND LYMPHOCYTE TRAFFIC, 2002, 512 : 203 - 208
[8] Simplified approach makes two-photon Multicolor imaging less costly
Franke, T., 1600, Laurin Publishing Co. Inc. (21):
[9] Multicolor fiber-optic two-photon endomicroscopy for brain imaging
Guan, Honghua
Liang, Wenxuan
Li, Ang
Gau, Yung-Tian A.
Chen, Defu
Li, Ming-Jun
Bergles, Dwight E.
Li, Xingde
OPTICS LETTERS, 2021, 46 (05) : 1093 - 1096
[10] Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS
Ueki, Hiroshi
Wang, I-Hsuan
Zhao, Dongming
Gunzer, Matthias
Kawaoka, Yoshihiro
NATURE PROTOCOLS, 2020, 15 (3) : 1041 - 1065

← 1 2 3 4 5 →