1,)2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA-AM) inhibits caffeine-induced apoptosis in human neuroblastoma cells

被引：6
作者：
Jang, MH
Shin, MC
Cho, YW
Baik, HH
Kim, SS
Hwang, EG
Kim, CJ
机构：
[1] Kyung Hee Univ, Coll Med, Dept Physiol, Seoul 130701, South Korea
[2] Kyung Hee Univ, Coll Med, Dept Biochem & Mol Biol, Seoul 130701, South Korea
[3] Kyung Hee Univ, Inst Global Environm, Seoul 130701, South Korea
[4] Konkuk Univ, Coll Med, Dept Thorac & Cardiovasc Surg, Chungju, South Korea
来源：
NEUROSCIENCE LETTERS | 2004年 / 358卷 / 03期
关键词：
caffeine; BAPTA-AM; apoptosis; neuroblastoma;
D O I：
10.1016/j.neulet.2004.01.040
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
In the present study, it was investigated whether 1.2-bis(2-aminophenoxy)ethane-N,N,N'N'-tetraacetic acid (BAPTA-AM), an intracellular Ca2+ chelator, possesses protective effect against caffeine-induced apoptosis in the central nervous system. Through morphological and biochemical analyses, cells treated with caffeine exhibited several apoptotic features. On the other hand, cells treated with caffeine and BAPTA-AM, showed decreased occurrence of apoptotic features. In addition, it was shown that BAPTA-AM treatment inhibits caffeine-induced increase of caspase-3 enzyme activity. These results show that caffeine induces apoptotic death in human SK-N-MC neuroblastoma cells and BAPTA-AM prevents apoptosis by attenuating caffeine-induced caspase-3 activation. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：189 / 192
页数：4
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