Combination therapy with polydeoxyribonucleotide and proton pump inhibitor enhances therapeutic effectiveness for gastric ulcer in rats

被引：26
作者：
Ko, Il-Gyu [1 ]
Kim, Sung-Eun [1 ]
Jin, Jun-Jang [1 ]
Hwang, Lakkyong [1 ]
Ji, Eun-Sang [1 ]
Kim, Chang-Ju [1 ]
Han, Jin-Hee [2 ]
Hong, In Taik [3 ]
Kwak, Min Seop [3 ]
Yoon, Jin Young [3 ]
Shin, Hyun Phil [3 ]
Jeon, Jung Won [3 ]
机构：
[1] Kyung Hee Univ, Coll Med, Dept Physiol, Seoul 02447, South Korea
[2] Kyung Hee Univ, Coll Med, Kyung Hee Med Ctr, Dept Anesthesiol & Pain Med, Seoul 02447, South Korea
[3] Kyung Hee Univ, Coll Med, Kyung Hee Univ Hosp Gangdong, Dept Internal Med, Seoul 05278, South Korea
来源：
LIFE SCIENCES | 2018年 / 203卷
关键词：
Gastric ulcer; Non-steroidal anti-inflammatory drugs; Proton pump inhibitor; Polydeoxyribonucleotide; Combination therapy; ADENOSINE RECEPTORS; ANGIOGENESIS; STIMULATION; INFLAMMATION; MECHANISM; DISEASE; ASPIRIN; INJURY; PDRN; VEGF;
D O I：
10.1016/j.lfs.2018.04.009
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Aims: The main action of proton pump inhibitors (PPIs) is to inhibit gastric acid secretion, and PPIs are widely used to treat gastric ulcer (GU). However, if the action of promoting gastric mucosal regeneration is added, the effectiveness of GU treatment can be enhanced. Thus, in order to improve the therapeutic effect on GU, we tried to develop combination therapy promoting regeneration in injured tissue besides suppressing gastric acid secretion. Main methods: Polydeoxyribonucleotide (PDRN) was selected to evaluate tissue regeneration, and pantoprazole was chosen as one of the PPIs. GU was induced by oral administration of indomethacin once a day for 7 days. Rats in drug-administered groups were intraperitoneally injected with 100 mu L normal saline, containing each drug at the indicated concentration, once a day for 14 days after inducing GU. Key findings: PDRN and PPI combination therapy potently improved tissue regeneration and inhibited production of pro-inflammatory cytokines. PDRN treatment with or without PPI increased the concentration of cyclic adenosine-3,5'-monophosphate (cAMP) and the ratio of phosphorylated cAMP response element-binding protein (p-CREB) to cAMP response element-binding protein (CREB). PDRN treatment with or without PPI also increased the expressions of vascular endothelial growth factor (VEGF) and adenosine A(2A) receptor. Significance: PDRN and PPI combination therapy showed more potent therapeutic effect on GU compared to the PDRN monotherapy or PPI monotherapy. The excellent therapeutic effect of PDRN and PPI combination therapy on GU appeared by promoting regeneration of damaged tissue as well as inhibiting gastric acid secretion.
引用
收藏
页码：12 / 19
页数：8
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