Analytical strategies for the characterization of therapeutic monoclonal antibodies

被引：113

作者：

Fekete, Szabolcs ^{[1
]}

Gassner, Anne-Laure ^{[1
]}

Rudaz, Serge ^{[1
]}

Schappler, Julie ^{[1
]}

Guillarme, Davy ^{[1
]}

机构：

[1] Univ Lausanne, Sch Pharmaceut Sci, Univ Geneva, CH-1211 Geneva 4, Switzerland

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2013年 / 42卷

关键词：

Adsorption; Analytical strategy; Capillary electrophoresis (CE); Characterization; Liquid chromatography (LC); Mass spectrometry; Monoclonal antibody (mAb); Recombinant; Separation; Therapeutic monoclonal antibody; CAPILLARY GEL-ELECTROPHORESIS; LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY; CHARGE HETEROGENEITY; POSTTRANSLATIONAL MODIFICATIONS; FLUORESCENCE DETECTION; MASS-SPECTROMETRY; QUALITY-CONTROL; CE; VALIDATION; PROTEINS;

D O I：

10.1016/j.trac.2012.09.012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Recombinant monoclonal antibodies (mAbs) have become particularly relevant for the treatment of autoimmune diseases or cancers. Because of their inherent complexity and for safety reasons, there is a need to develop powerful analytical methods to provide detailed characterizations of mAbs. The aim of the present review is to detail the state-of-the-art of analytical strategies for mAb characterization. It focuses on the most important separation techniques used in this field, specifically, the chromatographic and electrophoretic approaches and their combination with mass spectrometry (MS). Thanks to recent improvements in separation science and MS devices, mAbs can be analyzed more easily. However, there is still a need to find new approaches that avoid adsorption issues. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：74 / 83

页数：10

共 48 条

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