PCR-RFLP authentication of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus)

被引：86

作者：

Fajardo, V ^{[1
]}

González, I ^{[1
]}

López-Calleja, I ^{[1
]}

Martín, I ^{[1
]}

Hernández, PE ^{[1
]}

García, T ^{[1
]}

Martín, R ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2006年 / 54卷 / 04期

关键词：

game meat; species identification; 12S rRNA gene; PCR-RFLP;

D O I：

10.1021/jf051766r

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

PCR-RFLP analysis has been applied to the identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus). PCR amplification was carried out using a set of primers flanking a conserved region of similar to 712 base pairs from the mitochondrial 12S rRNA gene. Restriction site analysis based on sequence data from this DNA fragment permitted the selection of Msel, Mboll, BsA, and Apol endonucleases for species identification. The restriction profiles obtained when amplicons were digested with the chosen enzymes allowed the unequivocal identification of all domestic and game meat species analyzed in the present work.

引用

页码：1144 / 1150

页数：7

共 21 条

[1]

AVISE JC, 1987, ANNU REV ECOL SYST, V18, P489, DOI 10.1146/annurev.es.18.110187.002421

[2] Identifying unknown game species: experience with nucleotide sequencing of the mitochondrial cytochrome b gene and a subsequent basic local alignment search tool search [J].

Brodmann, PD ;

Nicholas, G ;

Schaltenbrand, P ;

Ilg, EC .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2001, 212 (04) :491-496

[3] Mammalian species identification by interspersed repeat PCR fingerprinting [J].

Buntjer, JB ;

Lenstra, JA .

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 1998, 21 (03) :121-127

[4]

Chen FC, 2000, J AOAC INT, V83, P79

[5] A note on the identification of Rupicapra rupicapra species by polymerase chain reaction product sequencing [J].

Colombo, F ;

Cardia, A ;

Renon, P ;

Cantoni, C .

MEAT SCIENCE, 2004, 66 (03) :753-755

[6] RAPD analysis of systematic relationships among the Cervidae [J].

Comincini, S ;

Sironi, M ;

Bandi, C ;

Giunta, C ;

Rubini, M ;

Fontana, F .

HEREDITY, 1996, 76 :215-221

[7] INTRASPECIFIC VARIATION IN MITOCHONDRIAL-DNA OF NORTH-AMERICAN CERVIDS [J].

CRONIN, MA .

JOURNAL OF MAMMALOGY, 1992, 73 (01) :70-82

[8] Sequence analysis of mitochondrial 12S rRNA gene can identify meat species [J].

Girish, PS ;

Anjaneyulu, ASR ;

Viswas, KN ;

Anand, M ;

Rajkumar, N ;

Shivakumar, BM ;

Bhaskar, S .

MEAT SCIENCE, 2004, 66 (03) :551-556

[9] Determination of the animal origin of raw food by species-specific PCR [J].

Herman, L .

JOURNAL OF DAIRY RESEARCH, 2001, 68 (03) :429-436

[10] DYNAMICS OF MITOCHONDRIAL-DNA EVOLUTION IN ANIMALS - AMPLIFICATION AND SEQUENCING WITH CONSERVED PRIMERS [J].

KOCHER, TD ;

THOMAS, WK ;

MEYER, A ;

EDWARDS, SV ;

PAABO, S ;

VILLABLANCA, FX ;

WILSON, AC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6196-6200

← 1 2 3 →