Anti-inflammatory activity of mushroom-derived hispidin through blocking of NF-B activation

BACKGROUNDHispidin, a polyphenol compound mainly derived from the valuable medicinal mushroom Phellinus species, has been found to possess distinct biological effects. However, the anti-inflammatory potential of hispidin still remains uncharacterized. RESULTSIn this study, the effects of hispidin on activation of nuclear factor kappa B (NF-B) and the subsequent production of inducible nitric oxide synthase (iNOS) were determined in the lipopolysaccharide (LPS)-induced macrophage RAW 264.7 cells. Our data indicated that hispidin inhibits transcriptional activity of NF-B in a dose-dependent manner. Hispidin also attenuated LPS-induced NF-B nuclear translocation and associated inhibitor of kappa B (IB-) degradation. Furthermore, hispidin deceased iNOS protein expression and the generation of reactive oxygen species (ROS) in the LPS-induced cells, but did not affect phosphorylation of mitogen-activated protein kinases. CONCLUSIONThese findings suggest that hispidin exhibits anti-inflammatory activity through suppressing ROS mediated NF-B pathway in mouse macrophage cells. (c) 2014 Society of Chemical Industry