Investigation of Fluorescence Resonance Energy Transfer between Fluorescein and Rhodamine 6G

被引:49
作者
Saha, Jaba [1 ]
Roy, Arpan Datta [1 ]
Dey, Dibyendu [1 ]
Chakraborty, Santanu [1 ]
Bhattacharjee, D. [1 ]
Paul, P. K. [2 ]
Hussain, Syed Arshad [1 ]
机构
[1] Tripura Univ, Dept Phys, Thin Film & Nanosci Lab, Suryamaninagar 799022, Tripura, India
[2] Jadavpur Univ, Dept Phys, Kolkata 700032, W Bengal, India
关键词
FRET; Dyes; Clay; pH sensor; INTRACELLULAR PH; SENSOR; PROBE; FRET; ION; LAPONITE; DESIGN; CARBON; MEDIA; CELLS;
D O I
10.1016/j.saa.2015.04.027
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
Fluorescence Resonance Energy Transfer between two organic dyes Fluorescein and Rhodamine 6G was investigated in aqueous solution in presence and absence of synthetic clay laponite. Spectroscopic studies suggest that both the dyes were present mainly as monomer in solution. Fluorescence Resonance Energy Transfer occurred from Fluorescein to Rhodamine 6G in solutions. Energy transfer efficiency increases in presence of laponite and the maximum efficiency was 72.00% in aqueous laponite dispersion. Energy transfer efficiency was found to be pH sensitive. It has been demonstrated that with proper calibration it is possible to use the present system under investigation to sense pH over a wide range from 1.5 to 8.0. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:143 / 149
页数:7
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