Separation of aromatic amines of MAK group, which are the reduction products of AZO dyes by partition HPLC chromatography

HPLC chromatographic systems allowing the separation and the determination of aromatic amines of MAK group, whose presence in textile products is prohibited, have been worked out. 29 aromatic amines were investigated. The application of C-18 columns and gradient of methanol-water [45 + 64 v/v for 12 min to 70 + 30 for 8 min and 80 + 20 v/v for 5 min to 85 + 15 for 10 min] with flow rate of 1 ml min(-1) allowed to separate and determine 17 of tested amines such as: 1,3-phenyldiamine, 2,4-diaminotoluene, 1,4-phenyldiamine, aniline, 4,4'-diaminediphenyl ether, o-toluidine, p.-toluidine, 4,4'-diaminodiphenylmethane, 2-amino-4-nitrotoluene, o-dianisidine, o-tolidin, anisole, 4-chloro-2-methylaniline, 2,4,6-trimethylaniline, 3,3'-dichlorobenzidine, diphenylamine, o-amino-azotoluene. Use of the mixture of methanol-water 53-47 v/v and flow rate 1.2 ml min(-1) allows to determine simultaneously 1,2-phenyldiamine, 4,4'-thiodianiline, benzidine, 1-naphtylamine, m-toluidine, 4-methoxy-1,3-phenylenediamine sulfate, 4,4'-diamino-3,3'-dimethyldiphenylmethane, 4,4'-methylene-bis(2-chloroaniline), 2,4,5-trimethylaniline. Measurements were performed with the use, of DAD spectrophotometric detector and fluorescence detector. Moreover, the identification of analyzed aromatic amines in samples of the following dyes was carried out: direct orange G. direct red 4B, direct blue 2B, folan red G, D Brown 95, D Blue 1 and coloured yam after reduction with artificial sweat.