Transgenic rabbit producing human growth hormone in milk

被引：0

作者：

Lipinski, Daniel ^{[1
,2
]}

Jura, Jolanta ^{[2
]}

Kalak, Robert ^{[1
]}

Plawski, Andrzej ^{[2
]}

Kala, Marta ^{[2
]}

Szalata, Marlena ^{[1
,2
]}

Jarmuz, Malgorzata ^{[2
]}

Korcz, Aleksandra ^{[4
]}

Slomska, Karolina ^{[1
]}

Jura, Jacek ^{[3
]}

Gronek, Piotr ^{[5
]}

Smorag, Zdzislaw ^{[3
]}

Pienkowski, Marek ^{[4
]}

Slomski, Ryszard ^{[1
,2
,4
]}

机构：

[1] Agr Univ Poznan, Dept Biochem & Biotechnol, PL-60637 Poznan, Poland

[2] Polish Acad Sci, Inst Human Genet, PL-60479 Poznan, Poland

[3] Natl Res Inst Anim Prod, Dept Anim Reprod, Balice, Poland

[4] PienGen Biomed Corp, Knoxville, TN USA

[5] Agr Univ Poznan, Dept Pig Breeding & Prod, PL-60637 Poznan, Poland

来源：

JOURNAL OF APPLIED GENETICS | 2003年 / 44卷 / 02期

关键词：

acidic protein promoter; human growth hormone (hGH); microinjection; milk; rat whey; transgenic rabbit;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene construct WAP(6xHisThr):hGH containing the entire human growth hormone gene (hGH) under the rat whey acidic protein (WAP) promoter regulating the expression in mammary glands of mammals was prepared. The 5' end of the gene was modified by the addition of a sequence encoding six histidine residues and a sequence recognized by thrombin. The gene construct was introduced by microinjection into the male pronucleus of a fertilized oocyte. The founder male rabbit was obtained with the transgene mapping to chromosome 7. The presence of the growth hormone was confirmed in samples of milk collected during the lactation of F1 generation females. The growth hormone can be easily purified by affinity chromatography and cleavage by thrombin to an active form.

引用

页码：165 / 174

页数：10

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