A one-step method for genetic transformation of non-piliated Neisseria meningitidis

被引：8
作者：
Bogdan, JA
Minetti, CASA
Blake, MS
机构：
[1] Baxter Healthcare Corp, Dept Mol Biol, Columbia, MD 21046 USA
[2] Rutgers State Univ, Dept Chem & Chem Biol, Piscataway, NJ 08855 USA
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2002年 / 49卷 / 01期
关键词：
genetic transformation; non-piliated; Neisseria meningitidis;
D O I：
10.1016/S0167-7012(01)00364-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A one-step method to chemically transform non-piliated Neisseria meningitidis strains previously resistant to conventional electrochemical transformation procedures has been developed. This method has been used to generate genetically engineered e meningococcal strains disrupted in the structural rmpM gene encoding Rmp. (C) 2002 Elsevier Science B.V. All rights reserved.
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页码：97 / 101
页数：5
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