Cloning, Expression, and Antigenicity of 14 Proteins from Campylobacter jejuni

被引：5

作者：

Zhang, Maojun ^{[1
,2
]}

Meng, Fanliang ^{[1
,2
]}

Cao, Fangfang ^{[1
,2
,3
]}

Qiao, Bo ^{[1
,2
,4
]}

Liu, Guodong ^{[1
,2
,5
]}

Liu, Hongying ^{[1
,2
]}

Zhou, Yizhuang ^{[1
,2
,6
]}

Dong, Haiyan ^{[1
,2
]}

Gu, Yixin ^{[1
,2
]}

Xiao, Di ^{[1
,2
]}

Zhang, Yongchan ^{[1
,2
]}

Zhang, Jianzhong ^{[1
,2
]}

机构：

[1] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Beijing 102206, Peoples R China

[2] Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China

[3] Peking Union Med Coll, Sch Publ Hlth, Beijing 100021, Peoples R China

[4] Zhengzhou Univ, Coll Publ Hlth, Zhengzhou, Henan, Peoples R China

[5] Beijing Municipal Ctr Dis Control & Prevent, Beijing, Peoples R China

[6] BGI, Beijing, Peoples R China

来源：

FOODBORNE PATHOGENS AND DISEASE | 2012年 / 9卷 / 08期

基金：

中国国家自然科学基金;

关键词：

GUILLAIN-BARRE-SYNDROME; OUTER-MEMBRANE PROTEIN; ACID EXTRACT; INFECTION; IDENTIFICATION; IMMUNOGENICITY; ASSOCIATION; ANTIBODIES; CHINA; MICE;

D O I：

10.1089/fpd.2011.1122

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Fourteen Campylobacter jejuni genes-porA, cadF, omp18, dnaK, flaC, peb1, peb2, peb3, peb4, ahpC, groEL, tuF, hipO, and Cj0069-were cloned and expressed in Escherichia coli BL21. The recombinant proteins were purified on histidine (His) and glutathione S-transferase (GST) trap columns using the AKTA Explorer 100 System. Recombinant proteins were visualized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The antigenicities of these recombinant proteins were assessed by Western blotting and enzyme-linked immunosorbent assays with anti-C. jejuni immune rabbit sera. Four recombinant proteins, including rGST-PorA, rHis-CadF, rGST-GroEL, and rGST-TuF, demonstrated reactions with both anti-serum and preimmune serum, while rHis-DnaK, rGST-FlaC, rGST-PEB2, rGST-PEB3, rGST-PEB4, and rGST-HipO showed variable antigenicity characteristics to the anti-sera derived from different C. jejuni strains. rHis-Omp18, rHis-PEB1, and rGST-AhpC demonstrated universal and specific antigenities with the entire anti-sera panel tested in this present study, while recombinant rGST-Cj0069 and rHis-DnaK did not react with any of the anti-C. jejuni sera tested. In conclusion, rGST-AhpC may be useful as a potential serodiagnostic antigen for C. jejuni infection.

引用

页码：706 / 712

页数：7

共 22 条

[1] Association between Campylobacter infection and Guillain-Barre syndrome [J].