Flow-injection method for the determination of serine using immobilized enzyme

被引：12
|
作者：
Yaqoob, M [1 ]
Nabi, A [1 ]
机构：
[1] Univ Balochristan, Dept Chem, Quetta, Pakistan
来源：
TALANTA | 2001年 / 55卷 / 06期
关键词：
serine; serine dehydratase; immobilized enzyme; flow-injection analysis; spectrophotometer;
D O I：
10.1016/S0039-9140(01)00528-8
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A flow-injection method for the determination of serine using a mini-column containing immobilized serine dehydratase isolated and purified from rat liver is described. Ammonia produced from the enzymatic reaction is coupled with hypochlorite and phenol in an alkaline medium yields a blue product due to the indophenol anion formation, which is the basis of a spectrophotometric detection at 640 nm. The limit of detection (2 x blank noise) is 0.01 mM with a sample throughput of 25 h(-1). Calibration graph is linear in the range 0.2-1.0 mM, with relative standard deviation 0.6-1.0%. (C) 2001 Elsevier Science B.V. All rights reserved.
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页码：1181 / 1186
页数：6
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