Effects of iloprost on bleomycin-induced pulmonary fibrosis in rats compared with methyl-prednisolone

被引:21
作者
Aytemur, Z. Ayfer [1 ]
Hacievliyagil, S. Savas [1 ]
Iraz, M. [2 ]
Samdanci, E. [3 ]
Ozerol, E. [4 ]
Kuku, I. [5 ]
Nurkabulov, Z. [3 ]
Yildiz, K. [3 ]
机构
[1] Inonu Univ, Fac Med, Dept Pulm Dis, Malatya, Turkey
[2] Bezmialem Univ, Fac Med, Dept Pharmacol Dis, Istanbul, Turkey
[3] Inonu Univ, Fac Med, Dept Pathol, Malatya, Turkey
[4] Inonu Univ, Fac Med, Dept Biochem, Malatya, Turkey
[5] Inonu Univ, Fac Med, Dept Haematol, Malatya, Turkey
关键词
Pulmonary fibrosis; Bleomycin; Iloprost; Methyl-prednisolone; Rats; LUNG FIBROSIS; PROSTACYCLIN; COLCHICINE; MICE;
D O I
10.1016/j.rppneu.2012.04.010
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Objective: Prostacyclin (PGI2) has been shown to inhibit the expression of pro-inflammatory and pro-fibrotic mediators in pulmonary fibrosis. In this study, we aimed to test the preventive effects of intraperitoneally administered iloprost, a stable PGI2 analog, on bleomycin-induced pulmonary fibrosis in rats and to compare the effects of iloprost with the effects of methylprednisolone, a traditional therapy. Methods: Rats were randomly allocated into four groups: 1. Saline alone (n = 6); 2. Bleomycin + placebo (n = 7); 3. Bleomycin + methyl-prednisolone (n = 7); 4. Bleomycin + iloprost (n = 7). Fibrotic changes in the lungs were demonstrated by analyzing the cellular composition of bronchoalveolar lavage fluid, histological evaluation and lung hydroxyproline content. Results: Fibrosis was made in the lungs of rats by bleomycin experimentally. Fibrosis scores in the methyl-prednisolone and the iloprost groups were significantly lower than in the placebo group (p < 0.05). Furthermore, the score of the iloprost group was significantly lower than the score of the methyl-prednisolone group. The hydroxyproline content was significantly less in the methyl-prednisolone and the iloprost groups (p < 0.05). In the placebo group, the neutrophil percentage in bronchoalveolar lavage was significantly higher than in the other groups, whereas the macrophage percentage in placebo group was significantly lower (p < 0.05). Conclusion: Iloprost has protective effect on the pulmonary fibrosis induced by bleomycin and it may be more effective in decreasing fibrotic changes than methyl-prednisolone. (C) 2011 Sociedade Portuguesa de Pneumologia. Published by Elsevier Espana, S. L. All rights reserved.
引用
收藏
页码:272 / 277
页数:6
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