Differential gene expression in leaves of a scab susceptible and a resistant apple cultivar upon Venturia inaequalis inoculation

被引：8

作者：

Holzapfel, Christine ^{[1
]}

Meisel, Barbara ^{[2
]}

Thuemmler, Fritz ^{[3
]}

Leser, Christoph ^{[1
]}

Treutter, Dieter ^{[1
]}

机构：

[1] Tech Univ Munich, Unit Fruit Sci, Ctr Life & Food Sci Weihenstephan, D-85354 Freising Weihenstephan, Germany

[2] Monsanto S Africa Pty Ltd, ZA-1512 Petit, South Africa

[3] Vertis Biotechnol AG, D-85354 Freising Weihenstephan, Germany

来源：

TREES-STRUCTURE AND FUNCTION | 2012年 / 26卷 / 01期

关键词：

Malus domestica; Venturia inaequalis; Differential gene expression; Lignification; CINNAMYL-ALCOHOL-DEHYDROGENASE; PATHOGENESIS-RELATED PROTEINS; MALUS-X-DOMESTICA; PHENOLIC-COMPOUNDS; INFECTION; DEFENSE; ELICITOR; LIGNIFICATION; ACCUMULATION; INDUCTION;

D O I：

10.1007/s00468-011-0663-6

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

Apple scab, one of the most damaging diseases in apple worldwide is caused by the fungal pathogen Venturia inaequalis. Pathogen-induced gene expression was analyzed in leaves of greenhouse-and field-grown trees of the scab susceptible cultivar Golden Delicious and the resistant cultivar Rewena using the macroarray technique. The results show that the defence of the Vf-resistant cultivar Rewena is based on different mechanisms than the basal defence response of the susceptible Golden Delicious: whereas lignification seems to play an essential role in scab defence of Rewena, a thaumatin-like as well as a flavonoid gene are assumed to be involved in the mostly insufficient defensive response of Golden Delicious. Furthermore, a method was developed for quantification of the V. inaequalis in infected leaves using real-time quantitative reverse transcription-PCR.

引用

页码：121 / 129

页数：9

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