Isolation and expression of the catA gene encoding the major vegetative catalase in Streptomyces coelicolor Muller

被引:25
|
作者
Cho, YH
Roe, JH
机构
[1] SEOUL NATL UNIV, COLL NAT SCI, DEPT MICROBIOL, SEOUL 151742, SOUTH KOREA
[2] SEOUL NATL UNIV, RES CTR MOL MICROBIOL, SEOUL 151742, SOUTH KOREA
关键词
D O I
10.1128/jb.179.12.4049-4052.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We isolated the catA gene for the major vegetative catalase from Streptomyces coelicolor Muller. It encodes a polypeptide of 488 residues (55,440 Da) that is highly homologous to typical monofunctional catalases. We investigated catA expression by analyzing both catA mRNA and catalase activity. catA expression was increased by H2O2 treatment but did not increase during stationary phase. A putative catalase (CatB) cross-reactive with anti-CatA antibody appeared during stationary phase and in the aerial mycelium.
引用
收藏
页码:4049 / 4052
页数:4
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