Aminopeptidase-N-independent entry of porcine epidemic diarrhea virus into Vero or porcine small intestine epithelial cells

被引：67

作者：

Ji, Chun-Miao ^{[1
]}

Wang, Bin ^{[1
]}

Zhou, Jiyong ^{[1
]}

Huang, Yao-Wei ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Anim Sci, Key Lab Anim Virol, Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China

来源：

VIROLOGY | 2018年 / 517卷

基金：

中国国家自然科学基金;

关键词：

Porcine epidemic diarrhea virus (PEDV); Aminopeptidase N (APN); Receptor; CRISPR/Cas9; Vero cells; IPEC-J2; cells; CORONAVIRUS; RECEPTOR; CHINA; PROPAGATION; INFECTION; CULTURE; SWINE; MODEL; LINE; PIGS;

D O I：

10.1016/j.virol.2018.02.019

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A monkey cell line Vero (ATCC CCL-81) is commonly used for porcine epidemic diarrhea virus (PEDV) propagation in vitro. However, it is still controversial whether the porcine aminopeptidase N (pAPN) counterpart on Vero cells (Vero-APN) confers PEDV entry. We found that endogenous expression of Vero-APN was undetectable in the mRNA and the protein levels in Vero cells. We cloned the partial Vero-APN gene (3340-bp) containing exons 1 to 9 from cellular DNA and subsequently generated two APN-knockout Vero cell lines by CRISPR/Cas9 approach. PEDV infection of two APN-knockout Vero cells had the same efficiency as the Vero cells with or without neuraminidase treatment. A Vero cells stably expressing pAPN did not increase PEDV production. SiRNA-knockdown of pAPN in porcine jejunum epithelial cells had no effects on PEDV infection. The results suggest that there exists an additional cellular receptor on Vero or porcine jejunal cells independent of APN for PEDV entry.

引用

页码：16 / 23

页数：8

共 30 条

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