Portable detection of clenbuterol using a smartphone-based electrochemical biosensor with electric field-driven acceleration

被引：49

作者：

Dou, Yanzhi ^{[1
,2
,3
]}

Jiang, Zhineng ^{[2
,3
]}

Deng, Wangping ^{[2
,3
]}

Su, Jing ^{[2
,3
]}

Chen, Shixing ^{[2
,3
]}

Song, Haiyun ^{[4
]}

Aldalbahi, Ali ^{[5
]}

Zuo, Xiaolei ^{[2
,3
]}

Song, Shiping ^{[2
,3
]}

Shi, Jiye ^{[6
]}

Fan, Chunhai ^{[2
,3
]}

机构：

[1] Nanjing Univ Posts & Telecommun, Natl Syngerst Innovat Ctr Adv Mat SICAM, IAM, KLOEID, 9 Wenyuan Rd, Nanjing 210023, Jiangsu, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China

[4] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Food Safety Res, Shanghai 200031, Peoples R China

[5] King Saud Univ, Dept Chem, Riyadh 11451, Saudi Arabia

[6] Univ Oxford, Kellogg Coll, Oxford OX2 6PN, England

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2016年 / 781卷

基金：

美国国家科学基金会;

关键词：

Electrochemistry; Portable; Sensor; Smartphone; Electrical field; GOLD NANOPARTICLES; SILVER DEPOSITION; IMMUNOSENSOR; IMMUNOASSAY; SENSOR; PROTEINS; PERFORMANCE; LABELS; AMPLIFICATION; ASSAY;

D O I：

10.1016/j.jelechem.2016.04.022

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have developed a fast, highly sensitive and low-cost biosensing system for the detection of clenbuterol (CLB), using a homemade mobile electrochemical device with an electric field-driven acceleration strategy. This system consists of an embedded circuit in smartphone for signal processing and a screen-printed carbon electrode (SPCE) modified with multi-walled carbon nanotubes (MWNTs) and goat anti mouse-immunoglobulin G (IgG) sensing layer (MWNTs-I-layer). CLB monoclonal antibody was assembled through its binding to the surface confined antibody. Such modified electrodes were used for rapid and sensitive amperometric immunosensing detection of CLB. Horseradish peroxidase-coupled CLB (CLB-HRP) competed with free CLB in the samples to bind the monoclonal antibody. By using this mobile system, we could detect CLB ranging from 03 ng.mL(-1) to 100 ng.mL(-1) with the detection limit of 0.076 ng.mL(-1). The whole competitive-type detection process was finished within 6 min. We expect this device can meet the requirements for field detection of various food security-related species. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：339 / 344

页数：6

共 34 条

[1] Sensitive and selective cocaine electrochemical detection using disposable sensors [J].