Production and hydrolytic efficiency ofenzymes from Trichoderma reesei RUTC30 using steam pretreated wheat straw as carbon source

被引:17
作者
Alvira, Pablo [1 ]
Gyalai-Korpos, Miklos [2 ]
Barta, Zsolt [2 ]
Miguel Oliva, Jose [1 ]
Reczey, Kati [2 ]
Ballesteros, Mercedes [1 ]
机构
[1] CIEMAT, Renewable Energies Div, Biofuels Unit, E-28040 Madrid, Spain
[2] Budapest Univ Technol & Econ, Dept Appl Biotechnol & Food Sci, Budapest, Hungary
关键词
cellulase production; hemicellulase; steam explosion; wheat straw; Trichoderma reesei; TRANSCRIPTIONAL REGULATION; ENZYMATIC-HYDROLYSIS; CELLULASE PRODUCTION; XYLANASE; FERMENTATION; SACCHARIFICATION; HEMICELLULASES; DEGRADATION; RUT-C30; ENZYMES;
D O I
10.1002/jctb.3955
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background Advances on enzyme technology have improved the economics of cellulosic ethanol. Nevertheless, there is still great interest to improve the production and the characteristics of the enzymatic preparations. One possibility to reduce the cost of the enzymes is on-site enzyme production in a biorefinery plant, in which part of the lignocellulosic material already available can be used as a cheap carbon source in the enzyme production. Results Steam exploded wheat straw at two different severity conditions was evaluated as carbon source for enzyme production by Trichoderma reesei. The results showed that volumetric production of cellulases and -glucosidase increased when using a carbon source with high cellulose content. When a carbon source with a higher content of hemicellulose was used the enzymatic mixture showed significantly higher proportions of hemicellulase activities (xylanase, -xylosidase and -L-arabinofuranosidase), which had a positive effect on subsequent enzymatic hydrolysis. Conclusions The proportion of cellulose and hemicellulose in the carbon source has an influence on the composition of the enzymatic cocktail. The higher the cellulose and hemicellulose content, the higher the titer of cellulase and hemicellulase produced by the fungus, respectively. The enzyme preparations with higher hemicellulase activities were more efficient in hydrolyzing xylan-containing substrates. (c) 2012 Society of Chemical Industry.
引用
收藏
页码:1150 / 1156
页数:7
相关论文
共 32 条
  • [1] Effect of endoxylanase and α-L-arabinofuranosidase supplementation on the enzymatic hydrolysis of steam exploded wheat straw
    Alvira, P.
    Negro, M. J.
    Ballesteros, M.
    [J]. BIORESOURCE TECHNOLOGY, 2011, 102 (06) : 4552 - 4558
  • [2] Strategies of Xylanase Supplementation for an Efficient Saccharification and Cofermentation Process from Pretreated Wheat Straw
    Alvira, Pablo
    Tomas-Pejo, Elia
    Jose Negro, Maria
    Ballesteros, Mercedes
    [J]. BIOTECHNOLOGY PROGRESS, 2011, 27 (04) : 944 - 950
  • [3] Transcriptional regulation of plant cell wall degradation by filamentous fungi
    Aro, N
    Pakula, T
    Penttilä, M
    [J]. FEMS MICROBIOLOGY REVIEWS, 2005, 29 (04) : 719 - 739
  • [4] INTERLABORATORY TESTING OF METHODS FOR ASSAY OF XYLANASE ACTIVITY
    BAILEY, MJ
    BIELY, P
    POUTANEN, K
    [J]. JOURNAL OF BIOTECHNOLOGY, 1992, 23 (03) : 257 - 270
  • [5] Ethanol production from steam-explosion pretreated wheat straw
    Ballesteros, Ignacio
    Negro, M. Jose
    Oliva, Jose Miguel
    Cabanas, Araceli
    Manzanares, Paloma
    Ballesteros, Mercedes
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2006, 130 (1-3) : 496 - 508
  • [6] MECHANISM OF ENZYMATIC CELLULOSE DEGRADATION - ISOLATION AND SOME PROPERTIES OF A BETA-GLUCOSIDASE FROM TRICHODERMA-VIRIDE
    BERGHEM, LER
    PETTERSSON, LG
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (02): : 295 - 305
  • [7] Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei
    Foreman, PK
    Brown, D
    Dankmeyer, L
    Dean, R
    Diener, S
    Dunn-Coleman, NS
    Goedegebuur, F
    Houfek, TD
    England, GJ
    Kelley, AS
    Meerman, HJ
    Mitchell, T
    Mitchinson, C
    Olivares, HA
    Teunissen, PJM
    Yao, J
    Ward, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (34) : 31988 - 31997
  • [8] Xylanase contribution to the efficiency of cellulose enzymatic hydrolysis of barley straw
    Garcia-Aparicio, Maria P.
    Ballesteros, Mercedes
    Manzanares, Paloma
    Ballesteros, Ignacio
    Gonzalez, Alerto
    Negro, M. Jose
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2007, 137 (1-12) : 353 - 365
  • [9] MEASUREMENT OF CELLULASE ACTIVITIES
    GHOSE, TK
    [J]. PURE AND APPLIED CHEMISTRY, 1987, 59 (02) : 257 - 268
  • [10] Removal of enzymatic and fermentation inhibitory compounds from biomass slurries for enhanced biorefinery process efficiencies
    Gurram, Raghu N.
    Datta, Saurav
    Lin, Yupo J.
    Snyder, Seth W.
    Menkhaus, Todd J.
    [J]. BIORESOURCE TECHNOLOGY, 2011, 102 (17) : 7850 - 7859