In vitro evaluation of antifungal activity of soybean (Glycine max) seed coat proteins

被引：7

作者：

Santos, P. O. ^{[1
]}

Santos, I. S. ^{[2
]}

Gomes, V. M. ^{[2
]}

Machado, O. L. T. ^{[1
]}

Fernandes, Kv. S. ^{[1
]}

Xavier-Filho, J. ^{[1
]}

Oliveira, A. E. A. ^{[1
]}

机构：

[1] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Quim & Funcao Proteinas & Peptideos, BR-28013602 Campos Dos Goytacazes, RJ, Brazil

[2] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Fisiol & Bioquim Microrganismos, BR-28013602 Campos Dos Goytacazes, RJ, Brazil

来源：

JOURNAL OF STORED PRODUCTS RESEARCH | 2008年 / 44卷 / 04期

关键词：

soybean; seed coat; phytopathogenic fungi;

D O I：

10.1016/j.jspr.2008.04.003

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Proteins in the soybean seed coat have previously been characterized; however, the function of these proteins is unknown. We show that a soybean seed coat protein fraction was able to inhibit the growth of Fusarium lateritium and Fusarium oxysporum phytopathogenic fungi. The antifungal fraction isolated by DEAE-Sepharose chromatography revealed the presence of peroxidase, vicilin and a 24 kDa protein homologous to acid phosphatases. Germination experiments revealed that both acid phosphatase and peroxidase were exuded during seed imbibition. We suggest that the set of seed coat antifungal proteins may help protect seeds from colonization by phytopathogenic fungi. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：310 / 315

页数：6

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