Extraction of polysaccharides from Phellinus nigricans mycelia and their antioxidant activities in vitro

被引：38

作者：

Wang, Zhanyong ^{[1
]}

Wang, Chenyu ^{[1
]}

Quan, Yue ^{[1
]}

机构：

[1] Liaoning Shihua Univ, Sch Environm & Biol Engn, Fushun 113001, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2014年 / 99卷

关键词：

Polysaccharides; Response surface methodology; Phellinus nigricans; Mycelia; Antioxidant; RESPONSE-SURFACE METHODOLOGY; ASSISTED EXTRACTION; OPTIMIZATION; ANTITUMOR;

D O I：

10.1016/j.carbpol.2013.08.073

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, response surface methodology was employed to optimize the extraction of polysaccharides from Phellinus nigricans mycelia. A central composite design was adopted to determine optimum parameters (extraction time, extraction temperature, extraction frequency, and ratio of water to raw material) that could yield a maximum polysaccharide. Results revealed the following optimum extraction conditions: extraction time, 2.8 h; ratio of water to raw material, 28; extraction frequency, 5; and extraction temperature, 95 degrees C. Under optimized conditions, the experimental yield of P. nigricans mycelia polysaccharides was 15.33 +/- 0.21%, which is consistent with the predicted yield. The antioxidant activity assay in vitro showed that the polysaccharides exhibited a high scavenging activity against superoxide anion, hydroxyl, and 1,1-diphenyl-2-picrylhydrazyl radicals. These polysaccharides also exhibited a strong reducing power. Thus, these polysaccharides can be used as natural antioxidants in functional foods or medicine. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：110 / 115

页数：6

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