Aphanizomenon gracile (Nostocales), a cylindrospermopsin-producing cyanobacterium in Polish lakes

被引:62
作者
Kokocinski, Mikolaj [1 ,2 ]
Mankiewicz-Boczek, Joanna [3 ]
Jurczak, Tomasz [4 ]
Spoof, Lisa [5 ]
Meriluoto, Jussi [5 ]
Rejmonczyk, Edyta [3 ]
Hautala, Henna [6 ]
Vehniainen, Markus [6 ]
Pawelczyk, Jakub [7 ]
Soininen, Janne [8 ]
机构
[1] Adam Mickiewicz Univ, Coll Polonicum, PL-69100 Slubice, Poland
[2] Adam Mickiewicz Univ, Dept Hydrobiol, Fac Biol, PL-61614 Poznan, Poland
[3] Polish Acad Sci, European Reg Ctr Ecohydrol UA UNESCO, PL-90364 Lodz, Poland
[4] Univ Lodz, Dept Appl Ecol, PL-90237 Lodz, Poland
[5] Abo Akad Univ, Dept Biosci, FIN-20520 Turku, Finland
[6] Univ Turku, Dept Biotechnol, FIN-20520 Turku, Finland
[7] Polish Acad Sci, Inst Med Biol, PL-93232 Lodz, Poland
[8] Univ Helsinki, Dept Geosci & Geog, FIN-00014 Helsinki, Finland
关键词
Aphanizomenon gracile; Cylindrospermopsis raciborskii; Cyanotoxins; Cylindrospermopsin; cyrA; cyrJ; pks; Toxicity; Lakes; RACIBORSKII NOSTOCALES; FRESH-WATER; TOXIN CYLINDROSPERMOPSIN; 1ST REPORT; SUBG; DOLICHOSPERMUM; PROTEIN-SYNTHESIS; GENUS ANABAENA; TOXICITY; OVALISPORUM; IDENTIFICATION;
D O I
10.1007/s11356-012-1426-7
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The cyanobacterial cytotoxin cylindrospermopsin (CYN) has become increasingly common in fresh waters worldwide. It was originally isolated from Cylindrospermopsis raciborskii in Australia; however, in European waters, its occurrence is associated with other cyanobacterial species belonging to the genera Aphanizomenon and Anabaena. Moreover, cylindrospermopsin-producing strains of widely distributed C. raciborskii have not yet been observed in European waters. The aims of this work were to assess the occurrence of CYN in lakes of western Poland and to identify the CYN producers. The ELISA tests, high-performance liquid chromatography (HPLC)-DAD, and HPLC-mass spectrometry (MS)/MS were conducted to assess the occurrence of CYN in 36 lakes. The cyrJ, cyrA, and pks genes were amplified to identify toxigenic genotypes of cyanobacteria that are capable of producing CYN. The toxicity and toxigenicity of the C. raciborskii and Aphanizomenon gracile strains isolated from the studied lakes were examined. Overall, CYN was detected in 13 lakes using HPLC-MS/MS, and its concentrations varied from trace levels to 3.0 mu g L-1. CYN was widely observed in lakes of western Poland during the whole summer under different environmental conditions. Mineral forms of nutrients and temperature were related to CYN production. The molecular studies confirmed the presence of toxigenic cyanobacterial populations in all of the samples where CYN was detected. The toxicity and toxigenicity analyses of isolated cyanobacteria strains revealed that A. gracile was the major producer of CYN.
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页码:5243 / 5264
页数:22
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