Increased glucose metabolic activity is associated with CD4 R T-cell activation and depletion during chronic HIV infection

Objectives:Glucose metabolism plays a fundamental role in supporting the growth, proliferation and effector functions of T cells. We investigated the impact of HIV infection on key processes that regulate glucose uptake and metabolism in primary CD4(+) and CD8(+) T cells.Design and methods:Thirty-eight HIV-infected treatment-naive, 35 HIV+/combination antiretroviral therapy, seven HIV+ long-term nonprogressors and 25 HIV control individuals were studied. Basal markers of glycolysis [e.g. glucose transporter-1 (Glut1) expression, glucose uptake, intracellular glucose-6-phosphate, and l-lactate] were measured in T cells. The cellular markers of immune activation, CD38 and HLA-DR, were measured by flow cytometry.Results:The surface expression of the Glut1 is up-regulated in CD4(+) T cells in HIV-infected patients compared with uninfected controls. The percentage of circulating CD4(+)Glut1(+) T cells was significantly increased in HIV-infected patients and was not restored to normal levels following combination antiretroviral therapy. Basal markers of glycolysis were significantly higher in CD4(+)Glut1(+) T cells compared to CD4(+)Glut1(-) T cells. The proportion of CD4(+)Glut1(+) T cells correlated positively with the expression of the cellular activation marker, HLA-DR, on total CD4(+) T cells, but inversely with the absolute CD4(+) T-cell count irrespective of HIV treatment status.Conclusion:Our data suggest that Glut1 is a potentially novel and functional marker of CD4(+) T-cell activation during HIV infection. In addition, Glut1 expression on CD4(+) T cells may be exploited as a prognostic marker for CD4(+) T-cell loss during HIV disease progression. (C) 2014 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins