Anxiolytic Effects of Herbal Ethanol Extract from Gynostemma pentaphyllum in Mice after Exposure to Chronic Stress

被引:25
作者
Choi, Hyun Sook [1 ,2 ]
Zhao, Ting Ting [1 ,2 ]
Shin, Keon Sung [1 ,2 ]
Kim, Seung Hwan [3 ]
Hwang, Bang Yeon [1 ,2 ]
Lee, Chong Kil [1 ,2 ]
Lee, Myung Koo [1 ,2 ]
机构
[1] Chungbuk Natl Univ, Coll Pharm, Dept Pharm, Chonju 361763, South Korea
[2] Chungbuk Natl Univ, Res Ctr Bioresource & Hlth, Chonju 361763, South Korea
[3] Kyung Hee Univ, Coll Phys Educ, Inst Sports Sci, Yongin 449701, South Korea
关键词
Gynostemma pentaphyllum; chronic stress; anxiety disorders; elevated plus-maze; marble burying; dopamine; serotonin; corticosterone; c-Fos; EARLY GENE-EXPRESSION; MESSENGER-RNA; RECEPTOR; CORTICOSTERONE; GYPENOSIDES; INDUCTION; BEHAVIOR; ANXIETY; MODEL; FOSB;
D O I
10.3390/molecules18044342
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, the effects of herbal ethanol extracts of Gynostemma pentaphyllum (GP-EX), on chronic electric footshock (EF) stress-induced anxiety disorders were investigated in mice, which were orally treated with GP-EX (30 mg/kg and 50 mg/kg) once a day for 14 days, followed by exposure to EF stress (2 mA, with an interval and duration of 10 s for 3 min). After the final exposure to EF stress, the elevated plus-maze and marble burying tests were performed, and the levels of dopamine and serotonin in the brain, the serum levels of corticosterone, and the expression of c-Fos in the paraventricular nuclei (PVN) were determined. Treatment with GP-EX (30 mg/kg and 50 mg/kg) significantly recovered the number of entries into open arms and time spent on open arms, which was reduced by chronic EF stress. GP-EX (30 mg/kg and 50 mg/kg) also reduced the number of marbles buried, which was increased by chronic EF stress. In addition, electric EF stress significantly decreased the levels of dopamine and serotonin in the brain, which was recovered by treatment with GP-EX (30 mg/kg and 50 mg/kg). The serum levels of corticosterone, which were markedly increased by chronic EF stress, were reduced by treatment with GP-EX (30 mg/kg and 50 mg/kg). Chronic EF stress-induced increases in c-Fos expression were also markedly reduced by GP-EX (30 mg/kg and 50 mg/kg) in the PVN. These results suggest that GP-EX shows anxiolytic functions, determined by the elevated plus-maze and marble burying tests, which are mediated by modulating the activity of dopamine and serotonin neurons as well as the expression of c-Fos in the brain, and the serum levels of corticosterone. Clinical trials of herbal GP-EX and its bioactive components need further investigation.
引用
收藏
页码:4342 / 4356
页数:15
相关论文
共 40 条
[1]   Effect of 5-{3-[((2S)-1,4-benzodioxan-2-ylmethyl)amino]propoxy}-1,3-benzodioxole HCl (MKC-242), a novel 5-HT1A-receptor agonist, on aggressive behavior and marble burying behavior in mice [J].
Abe, M ;
Nakai, H ;
Tabata, R ;
Saito, K ;
Egawa, M .
JAPANESE JOURNAL OF PHARMACOLOGY, 1998, 76 (03) :297-304
[2]   RECEPTOR ACTIVITY AND TURNOVER OF DOPAMINE AND NORADRENALINE AFTER NEUROLEPTICS [J].
ANDEN, NE ;
BUTCHER, SG ;
CORRODI, H ;
FUXE, K ;
UNGERSTEDT, U .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1970, 11 (03) :303-+
[3]  
[Anonymous], PHYTOCHEM REV
[4]   Chronic toxicity of Gynostemma pentaphyllum [J].
Attawish, A ;
Chivapat, S ;
Phadungpat, S ;
Bansiddhi, J ;
Techadamrongsin, Y ;
Mitrijit, O ;
Chaorai, B ;
Chavalittumrong, P .
FITOTERAPIA, 2004, 75 (06) :539-551
[5]   Induction of ΔFosB in the periaqueductal gray by stress promotes active coping responses [J].
Berton, Olivier ;
Covington, Herbert E., III ;
Ebner, Karl ;
Tsankova, Nadia M. ;
Carle, Tiffany L. ;
Ulery, Paula ;
Bhonsle, Akshay ;
Barrot, Michel ;
Krishnan, Vaishnav ;
Singewald, Georg M. ;
Singewald, Nicolas ;
Birnbaum, Shari ;
Neve, Rachael L. ;
Nestler, Eric J. .
NEURON, 2007, 55 (02) :289-300
[6]   MAJOR TRANQUILIZERS CAN BE DISTINGUISHED FROM MINOR TRANQUILIZERS ON THE BASIS OF EFFECTS ON MARBLE BURYING AND SWIM-INDUCED GROOMING IN MICE [J].
BROEKKAMP, CL ;
RIJK, HW ;
JOLYGELOUIN, D ;
LLOYD, KL .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1986, 126 (03) :223-229
[7]   Pharmacological studies on the anxiolytic effect of standardized Schisandra lignans extract on restraint-stressed mice [J].
Chen, Wai-Wei ;
He, Rong-Rong ;
Li, Yi-Fang ;
Li, Shan-Bing ;
Tsoi, Bun ;
Kurihara, Hiroshi .
PHYTOMEDICINE, 2011, 18 (13) :1144-1147
[8]   Neuroprotective Effects of Herbal Ethanol Extracts from Gynostemma pentaphyllum in the 6-Hydroxydopamine-Lesioned Rat Model of Parkinson's Disease [J].
Choi, Hyun Sook ;
Park, Mi Sook ;
Kim, Seung Hwan ;
Hwang, Bang Yeon ;
Lee, Chong Kil ;
Lee, Myung Koo .
MOLECULES, 2010, 15 (04) :2814-2824
[9]   Differential behavioral effects of the antidepressants reboxetine, fluoxetine, and moclobemide in a modified forced swim test following chronic treatment [J].
Cryan, JF ;
Page, ME ;
Lucki, I .
PSYCHOPHARMACOLOGY, 2005, 182 (03) :335-344
[10]   Chronic stress regulates levels of mRNA transcripts encoding β subunits of the GABAA receptor in the rat stress axis [J].
Cullinan, WE ;
Wolfe, TJ .
BRAIN RESEARCH, 2000, 887 (01) :118-124