Comparison of PNA probe-based real-time PCR and Cobas TaqMan MTB for detection of MTBC

被引：10

作者：

Kim, Jae Woo ^{[1
]}

Choi, Young Jin ^{[2
]}

Kim, Hwi Jun ^{[2
]}

Park, Joon Soo ^{[3
]}

Nam, Hae Seon ^{[4
]}

Hwangbo, Young ^{[5
]}

Kim, Doug Uk ^{[6
]}

Park, Kwi Sung ^{[6
]}

机构：

[1] Soonchunhyang Univ, Coll Med, Dept Emergency Med, Cheonan 330090, South Korea

[2] Soonchunhyang Univ, Coll Med, Dept Lab Med, Cheonan 330090, South Korea

[3] Soonchunhyang Univ, Coll Med, Dept Pediat, Cheonan 330090, South Korea

[4] Soonchunhyang Univ, Coll Med, Dept Parasitol, Cheonan 330090, South Korea

[5] Soonchunhyang Univ, Coll Med, Dept Prevent Med, Cheonan 330090, South Korea

[6] Chungcheongnam Do Hlth & Environm Res Inst, Taejon 300801, South Korea

来源：

BIOCHIP JOURNAL | 2013年 / 7卷 / 02期

关键词：

Peptide nucleic acids; PCR; Mycobacterium tuberculosis complex; Tuberculosis; Real-time PCR; MYCOBACTERIUM-TUBERCULOSIS COMPLEX; DIAGNOSIS; TESTS;

D O I：

10.1007/s13206-013-7201-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We compared the sensitivities and specificities of peptide nucleic acid (PNA) probe-based real-time PCR (PNAqPCR (TM) TB/NTM) and Cobas TaqMan MTB assays for detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens. A total of 425 clinical specimens including 360 respiratory specimens and 65 non-respiratory specimens were evaluated for comparative analysis. In respiratory specimens, the sensitivity of TaqMan MTB and PNAqPCR assay for detection of MTBC was 82.9% and 91.5%, respectively. In non-respiratory specimens, the sensitivity of the TaqMan MTB and PNAqPCR assay was 23.1% and 76.9%, respectively. Overall, the sensitivity and specificity of the TaqMan MTB assay for detection of MTBC was 76.9% and 100%, respectively. The PNAqPCR assay had a sensitivity and specificity of 90% and 99.7%, respectively.

引用

页码：85 / 88

页数：4

共 14 条

[1] [Anonymous], 2011, Global Health and Aging
[2] Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli
Behr, MA
Warren, SA
Salamon, H
Hopewell, PC
de Leon, AP
Daley, CL
Small, PM
[J]. LANCET, 1999, 353 (9151) : 444 - 449
[3] Use of Roche AMPLICOR Mycobacterium tuberculosis PCR in early diagnosis of tuberculous meningitis
Bonington, A
Strang, JIG
Klapper, PE
Hood, SV
Rubombora, W
Penny, M
Willers, R
Wilkins, EGL
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (05) : 1251 - 1254
[4] Comparison of Two Molecular Methods for Rapid Diagnosis of Extrapulmonary Tuberculosis
Causse, Manuel
Ruiz, Pilar
Bautista Gutierrez-Aroca, Juan
Casal, Manuel
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (08) : 3065 - 3067
[5] Evaluation of Peptide Nucleic Acid Probe-based Real-time PCR for Detection of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria in Respiratory Specimens
Choi, Young Jin
Kim, Hwi Jun
Shin, Hee Bong
Nam, Hae Seon
Lee, Sang Han
Park, Joon Soo
Park, Kwi Sung
Baek, Kyoung Ah
[J]. ANNALS OF LABORATORY MEDICINE, 2012, 32 (04) : 257 - 263
[6] Evaluation of peptide nucleic acid array for the detection of hepatitis B virus mutations associated with antiviral resistance
Choi, Young Jin
Kim, Hong Soo
Lee, Sae Hwan
Park, Joon Soo
Hae Seon Nam
Kim, Hwi Jun
Kim, Chang Jin
Jeong, Dong Jun
Park, Kwi Sung
Baek, Kyoung Ah
[J]. ARCHIVES OF VIROLOGY, 2011, 156 (09) : 1517 - 1524
[7] A systematic review of rapid diagnostic tests for the detection of tuberculosis infection
Dinnes, J.
Deeks, J.
Kunst, H.
Gibson, A.
Cummins, E.
Waugh, N.
Drobniewski, F.
Lalvani, A.
[J]. HEALTH TECHNOLOGY ASSESSMENT, 2007, 11 (03) : 1 - +
[8] Current evidence on diagnostic accuracy of commercially based nucleic acid amplification tests for the diagnosis of pulmonary tuberculosis
Greco, S.
Girardi, E.
Navarra, A.
Saltini, C.
[J]. THORAX, 2006, 61 (09) : 783 - 790
[9] Higuchi R, 2011, MOLECULAR MICROBIOLOGY: DIAGNOSTIC PRINCIPLES AND PRACTICE, SECOND EDITION, P301
[10] Evaluation of Cobas TaqMan MTB PCR for Detection of Mycobacterium tuberculosis
Kim, Jeong Hyun
Kim, Young Jae
Ki, Chang-Seok
Kim, Ji-Youn
Lee, Nam Yong
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (01) : 173 - 176

← 1 2 →