Comparison of PNA probe-based real-time PCR and Cobas TaqMan MTB for detection of MTBC

被引：10

作者：

Kim, Jae Woo ^{[1
]}

Choi, Young Jin ^{[2
]}

Kim, Hwi Jun ^{[2
]}

Park, Joon Soo ^{[3
]}

Nam, Hae Seon ^{[4
]}

Hwangbo, Young ^{[5
]}

Kim, Doug Uk ^{[6
]}

Park, Kwi Sung ^{[6
]}

机构：

[1] Soonchunhyang Univ, Coll Med, Dept Emergency Med, Cheonan 330090, South Korea

[2] Soonchunhyang Univ, Coll Med, Dept Lab Med, Cheonan 330090, South Korea

[3] Soonchunhyang Univ, Coll Med, Dept Pediat, Cheonan 330090, South Korea

[4] Soonchunhyang Univ, Coll Med, Dept Parasitol, Cheonan 330090, South Korea

[5] Soonchunhyang Univ, Coll Med, Dept Prevent Med, Cheonan 330090, South Korea

[6] Chungcheongnam Do Hlth & Environm Res Inst, Taejon 300801, South Korea

来源：

BIOCHIP JOURNAL | 2013年 / 7卷 / 02期

关键词：

Peptide nucleic acids; PCR; Mycobacterium tuberculosis complex; Tuberculosis; Real-time PCR; MYCOBACTERIUM-TUBERCULOSIS COMPLEX; DIAGNOSIS; TESTS;

D O I：

10.1007/s13206-013-7201-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We compared the sensitivities and specificities of peptide nucleic acid (PNA) probe-based real-time PCR (PNAqPCR (TM) TB/NTM) and Cobas TaqMan MTB assays for detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens. A total of 425 clinical specimens including 360 respiratory specimens and 65 non-respiratory specimens were evaluated for comparative analysis. In respiratory specimens, the sensitivity of TaqMan MTB and PNAqPCR assay for detection of MTBC was 82.9% and 91.5%, respectively. In non-respiratory specimens, the sensitivity of the TaqMan MTB and PNAqPCR assay was 23.1% and 76.9%, respectively. Overall, the sensitivity and specificity of the TaqMan MTB assay for detection of MTBC was 76.9% and 100%, respectively. The PNAqPCR assay had a sensitivity and specificity of 90% and 99.7%, respectively.

引用

页码：85 / 88

页数：4

共 14 条

[1]

[Anonymous], 2011, Global Health and Aging

[2] Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli [J].

Behr, MA ;

Warren, SA ;

Salamon, H ;

Hopewell, PC ;

de Leon, AP ;

Daley, CL ;

Small, PM .

LANCET, 1999, 353 (9151) :444-449

[3] Use of Roche AMPLICOR Mycobacterium tuberculosis PCR in early diagnosis of tuberculous meningitis [J].

Bonington, A ;

Strang, JIG ;

Klapper, PE ;

Hood, SV ;

Rubombora, W ;

Penny, M ;

Willers, R ;

Wilkins, EGL .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (05) :1251-1254

[4] Comparison of Two Molecular Methods for Rapid Diagnosis of Extrapulmonary Tuberculosis [J].

Causse, Manuel ;

Ruiz, Pilar ;

Bautista Gutierrez-Aroca, Juan ;

Casal, Manuel .

JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (08) :3065-3067

[5] Evaluation of Peptide Nucleic Acid Probe-based Real-time PCR for Detection of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria in Respiratory Specimens [J].

Choi, Young Jin ;

Kim, Hwi Jun ;

Shin, Hee Bong ;

Nam, Hae Seon ;

Lee, Sang Han ;

Park, Joon Soo ;

Park, Kwi Sung ;

Baek, Kyoung Ah .

ANNALS OF LABORATORY MEDICINE, 2012, 32 (04) :257-263

[6] Evaluation of peptide nucleic acid array for the detection of hepatitis B virus mutations associated with antiviral resistance [J].

Choi, Young Jin ;

Kim, Hong Soo ;

Lee, Sae Hwan ;

Park, Joon Soo ;

Hae Seon Nam ;

Kim, Hwi Jun ;

Kim, Chang Jin ;

Jeong, Dong Jun ;

Park, Kwi Sung ;

Baek, Kyoung Ah .

ARCHIVES OF VIROLOGY, 2011, 156 (09) :1517-1524

[7] A systematic review of rapid diagnostic tests for the detection of tuberculosis infection [J].

Dinnes, J. ;

Deeks, J. ;

Kunst, H. ;

Gibson, A. ;

Cummins, E. ;

Waugh, N. ;

Drobniewski, F. ;

Lalvani, A. .

HEALTH TECHNOLOGY ASSESSMENT, 2007, 11 (03) :1-+

[8] Current evidence on diagnostic accuracy of commercially based nucleic acid amplification tests for the diagnosis of pulmonary tuberculosis [J].

Greco, S. ;

Girardi, E. ;

Navarra, A. ;

Saltini, C. .

THORAX, 2006, 61 (09) :783-790

[9]

Higuchi R, 2011, MOLECULAR MICROBIOLOGY: DIAGNOSTIC PRINCIPLES AND PRACTICE, SECOND EDITION, P301

[10] Evaluation of Cobas TaqMan MTB PCR for Detection of Mycobacterium tuberculosis [J].

Kim, Jeong Hyun ;

Kim, Young Jae ;

Ki, Chang-Seok ;

Kim, Ji-Youn ;

Lee, Nam Yong .

JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (01) :173-176

← 1 2 →