Assessment of DNA complexation onto polyelectrolyte-coated magnetic silica nanoparticles

被引：16

作者：

Davila-Ibanez, Ana B. ^{[1
]}

Buurma, Niklaas J. ^{[2
]}

Salgueirino, Veronica ^{[1
]}

机构：

[1] Univ Vigo, Dept Fis Aplicada, Vigo 36310, Spain

[2] Cardiff Univ, Phys Organ Chem Ctr, Sch Chem, Cardiff CF10 3AX, S Glam, Wales

来源：

NANOSCALE | 2013年 / 5卷 / 11期

基金：

英国工程与自然科学研究理事会;

关键词：

ISOTHERMAL TITRATION CALORIMETRY; IRON-OXIDE NANOPARTICLES; WATER-SALT SOLUTIONS; BINDING; THERMODYNAMICS; MULTILAYERS; PHASE; ASSOCIATION; CONDENSATION; CYTOTOXICITY;

D O I：

10.1039/c3nr34358h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The polyelectrolyte-DNA complexation method to form magnetoplexes using silica-coated iron oxide magnetic nanoparticles as inorganic substrates is an attractive and promising process in view of the potential applications including magnetofection, DNA extraction and purification, and directed assembly of nanostructures. Herein, we present a systematic physico-chemical study that provides clear evidence of the type of interactions established, reflects the importance of the DNA length, the nanoparticle size and the ionic strength, and permits the identification of the parameters controlling both the stability and the type of magnetoplexes formed. This information can be used to develop targeted systems with properties optimized for the various proposed applications of magnetoplexes.

引用

页码：4797 / 4807

页数：11

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