Survivin Down-regulation by α-Santalol Is Not Mediated Through PI3K-AKT Pathway in Human Breast Cancer Cells

被引:1
作者
Bommareddy, Ajay [1 ]
Crisamore, Karryn [1 ]
Fillman, Sarah [1 ]
Brozena, Sarah [1 ]
Steigerwalt, James [1 ]
Landis, Terra [1 ]
Vanwert, Adam L. [1 ]
Dwivedi, Chandradhar [2 ]
机构
[1] Wilkes Univ, Nesbitt Sch Pharm, Dept Pharmaceut Sci, Wilkes Barre, PA 18766 USA
[2] S Dakota State Univ, Coll Pharm, Dept Pharmaceut Sci, Brookings, SD 57007 USA
关键词
alpha-santalol; survivin; caspase-3; PI3K-AKT pathway; SKIN TUMOR-DEVELOPMENT; RESISTANCE; EXPRESSION; SYNERGIZES; NUTLIN-3; WOMEN;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: alpha-Santalol, a terpenoid found in sandalwood oil, has been shown to inhibit cancer cell growth in vitro by inducing apoptosis. This study was performed to investigate the anticancer properties of alpha-santalol associated with the induction of apoptosis in cultured MCF-7 [estrogen receptor (ER)-positive, and wild-type p53)] and MDA-MB231 (ER-negative and mutant p53) breast cancer cells. Materials and Methods: Expression of major proteins examined in the study were determined using a standard western blot protocol and analyzed by LICOR-Odyssey infrared scanner. Total protein levels of survivin were confirmed by survivin enzyme-linked immunosorbent assay (ELISA) kit. Cell viability was assessed by the trypan blue dye exclusion assay, and caspase-3 activity was determined by caspase-3 (active) ELISA kit. Results: Treatment of breast cancer cells for 6 and 9 h with alpha-santalol (20, and 40 mu M) resulted in statistically significant concentration-dependent down-regulation of survivin. Phosphorylated protein kinase B (pAKT) levels were found to be slightly up-regulated despite the down-regulation of survivin. Pharmacological inhibition of the phosphoinositide 3-kinase - protein kinase B (PI3K-AKT) pathway did not result in a synergistic/additive increase in cell death or caspase-3 activity caused by alpha-santalol. Conclusion: The study reveals that survivin down-regulation by alpha-santalol in breast cancer cells is not mediated through the PI3K-AKT pathway.
引用
收藏
页码:5353 / 5357
页数:5
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