Purification, crystallization and preliminary crystallographic analysis of a dissimilatory DsrAB sulfite reductase in complex with DsrC

被引：11

作者：

Oliveira, Tania F. ^{[1
]}

Vonrhein, Clemens ^{[2
]}

Matias, Pedro M. ^{[1
]}

Venceslau, Sofia S. ^{[1
]}

Pereira, Ines A. C. ^{[1
]}

Archer, Margarida ^{[1
]}

机构：

[1] Univ Nova Lisboa, ITQB UNL, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal

[2] Global Phasing Ltd, Cambridge CB3 0AX, England

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2008年 / 164卷 / 02期

关键词：

Crystallization; MAD phasing; Dissimilatory sulfite reductase; DsrABC complex;

D O I：

10.1016/j.jsb.2008.07.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dissimilatory sulfite reductase (dSiR, DsrAB) is a key protein in dissimilatory sulfur metabolism, one of the earliest types of energy metabolism to be traced on earth. dSirs are large oligomeric proteins around 200 kDa forming an alpha(2)beta(2) arrangement and including a unique siroheme-[4Fe-4S] coupled cofactor. Here, we report the purification, crystallization and preliminary X-ray diffraction analysis of dSir isolated from Desulfovibrio vulgaris Hildenborough, also known as desulfoviridin. In this enzyme the DsrAB protein is associated with DsrC, a protein of unknown function that is believed to play an important role in the sulfite reduction. Crystals belong to the monoclinic space group P2(1) with unit-cell parameters a = 122.7, b = 119.4 and c = 146.7 angstrom and beta =110.0 degrees, and diffract X-rays to 2.8 angstrom on a synchrotron source. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：236 / 239

页数：4

共 35 条

[1] Methods used in the structure determination of bovine mitochondrial F-1 ATPase [J].

Abrahams, JP ;

Leslie, AGW .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1996, 52 :30-42

[2] THE DISSIMILATORY SULFITE REDUCTASE FROM DESULFOSARCINA-VARIABILIS IS A DESULFORUBIDIN CONTAINING UNCOUPLED METALATED SIROHEMES AND S = 9/2 IRON-SULFUR CLUSTERS [J].

ARENDSEN, AF ;

VERHAGEN, MFJM ;

WOLBERT, RBG ;

PIERIK, AJ ;

STAMS, AJM ;

JETTEN, MSM ;

HAGEN, WR .

BIOCHEMISTRY, 1993, 32 (39) :10323-10330

[3] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[4]

Cowtan K., 1994, JOINT CCP4 ESF EACBM, V31, P34

[5] The Buccaneer software for automated model building.: 1.: Tracing protein chains [J].

Cowtan, Kevin .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2006, 62 :1002-1011

[6] The relationship between structure and function for the sulfite reductases [J].

Crane, BR ;

Getzoff, ED .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1996, 6 (06) :744-756

[7] SULFITE REDUCTASE STRUCTURE AT 1.6 ANGSTROM - EVOLUTION AND CATALYSIS FOR REDUCTION OF INORGANIC ANIONS [J].

CRANE, BR ;

SIEGEL, LM ;

GETZOFF, ED .

SCIENCE, 1995, 270 (5233) :59-67

[8] Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods [J].

delaFortelle, E ;

Bricogne, G .

MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :472-494

[9] Domain evolution and functional diversification of sulfite reductases [J].

Dhillon, A ;

Goswami, S ;

Riley, M ;

Teske, A ;

Sogin, M .

ASTROBIOLOGY, 2005, 5 (01) :18-29

[10] Scaling and assessment of data quality [J].

Evans, P .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2006, 62 :72-82

← 1 2 3 4 →