Qualitative and quantitative analysis of thermosensitive liposomes loaded with doxorubicin

被引：2
|
作者：
Tazina, E. V. [1 ]
Ignatieva, E. V. [1 ]
Polozkova, A. P. [1 ]
Oborotova, N. A. [1 ]
机构：
[1] Russian Acad Med Sci, Blokhin Russian Canc Res Ctr, Moscow, Russia
来源：
PHARMACEUTICAL CHEMISTRY JOURNAL | 2012年 / 46卷 / 01期
关键词：
thermosensitive liposomes; doxorubicin; lipids; sucrose; TLC; spectrophotometry; validation; TEMPERATURE SENSITIVE LIPOSOMES; DRUG-RELEASE; STABILITY;
D O I：
10.1007/s11094-012-0733-0
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Chromatographic and spectrophotometric methods for the qualitative and quantitative analysis of thermosensitive liposomes loaded with doxorubicin were developed. Lipids and doxorubicin in thermosensitive liposomes were separated by TLC using the two solvent systems CHCl3:MeOH:NH4OH (conc.) (65:25:4) and CHCl3:MeOH:HOAc (glacial):H2O (25:15:4:2). Chromatographic determination of sucrose in thermosensitive liposomes was performed using the solvent systems 1,2-dichloroethane:HOAc (anhydrous): MeOH:H2O (10:5:3:2) or -PrOH:Me2CO:Et2O:H2O (7:7:2:4). The spectrophotometric assay for doxorubicin in thermosensitive liposomes was validated.
引用
收藏
页码：54 / 59
页数：6
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