Human umbilical cord blood progenitors: The potential of these hematopoietic cells to become neural

被引:83
|
作者
Chen, N
Hudson, JE
Walczak, P
Misiuta, I
Garbuzova-Davis, S
Jiang, LX
Sanchez-Ramos, J
Sanberg, PR
Zigova, T
Willing, AE
机构
[1] Univ S Florida, Coll Med, Dept Neurosurg, Ctr Excellence Aging & Brain Repair, Tampa, FL 33612 USA
[2] Univ S Florida, Coll Med, Dept Neurosurg, Tampa, FL 33612 USA
[3] Univ S Florida, Coll Med, Dept Neurol, Tampa, FL 33612 USA
[4] Univ S Florida, Coll Med, Dept Anat, Tampa, FL 33612 USA
[5] Univ S Florida, Coll Med, Dept Psychiat, Tampa, FL 33612 USA
[6] Univ S Florida, Coll Med, Dept Pharmacol & Therapeut, Tampa, FL 33612 USA
[7] Univ S Florida, Coll Med, Dept Pathol, Tampa, FL 33612 USA
[8] James A Haley Vet Hosp, Tampa, FL 33612 USA
关键词
human; umbilical cord blood; stem/progenitor cell; multipotential differentiation; hematopoietic and neural antigens; neuropoiesis;
D O I
10.1634/stemcells.2004-0284
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The mononuclear fraction from human umbilical cord blood (HUCB) contains a significant number of stem/progenitor cells that in theory could become any cell in the body, including neurons. Taking into consideration that transdifferentiation would be a very rare event and also knowing that overlapping genetic programs for hematopoiesis and neuropoiesis exist, we undertook a characterization of the HUCB mononuclear fraction, including analysis of cellular subpopulations and their morphology, cell viability, proliferation, and expression of neural and hematopoietic antigens. Two cell populations were apparent-adherent and floating fractions. The adherent fraction was mainly lymphocytes (similar to 53%) expressing hematopoietic antigens. Upon replate, the floating population had many cells that expressed stem cell antigens. More of the cells in this subfraction expressed neural proteins. Neurotrophin receptors trkB and trkC were present in both cell fractions, although expression was higher in the floating fraction. Our initial characterization suggests that a subpopulation of cells exists within the HUCB mononuclear fraction that seems to have the potential to become neural cells, which could then be used in the development of cell-based therapies for brain injuries and diseases. STEM CELLS 2005,23:1560-1570.
引用
收藏
页码:1560 / 1570
页数:11
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