Simultaneous Determination of Human Enterovirus 71 and Coxsackievirus B3 by Dual-Color Quantum Dots and Homogeneous Immunoassay

被引:118
作者
Chen, Lu [1 ]
Zhang, Xiaowei [2 ]
Zhou, Guohua [1 ]
Xiang, Xia [1 ]
Ji, Xinghu [1 ]
Zheng, Zhenhua [2 ]
He, Zhike [1 ]
Wang, Hanzhong [2 ]
机构
[1] Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China
[2] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
基金
美国国家科学基金会; 中国国家自然科学基金;
关键词
RESONANCE ENERGY-TRANSFER; GRAPHENE OXIDE; DNA; QDS; FLUORESCENCE; CONJUGATION; ANTIBODIES;
D O I
10.1021/ac203172x
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Human Enterovirus 71 (EV71) and Coxsackievirus B3 (CVB3) have high risks for morbidity and mortality. A virus quantitation immunoassay has been proposed by employing two colored quantum dots (QDs), antibodies of the virus, and graphene oxide (GO). The QDs are streptavidin-conjugated quantum dots (SA-QDs), and the antibodies are biotinylated antibodies. Biotinylated EV71 antibody (Ab1) was associated with 525 nm green colored SA-QDs via biotinstreptavidin interaction forming QDs-Ab1, whereas biotinylated CVB3 antibody (Ab2) was associated with 605 nm red colored SA-QDs via biotin streptavidin interaction forming QDs-Ab2. GO was an excellent quencher to the fluorescence of both QDs-Ab1 and QDs-Ab2. The targets of EV71 and CVB3 can break up the complex of QDs-Ab and GO, recovering the fluorescence of QDs-Ab1 and QDs-Ab2, respectively. Using these two different colored QDs-Ab fluorescence recovery intensities upon the addition of targets EV71 and CVB3, the two enteroviruses can be simultaneously quantitatively determined with a single excitation light. The detection limits of EV71 and CVB3 are 0.42 and 0.39 ng mL(-1) based on 3 times signal-to-noise ratio, respectively. More importantly, this strategy can be further used as a universal method for any protein or virus determination by changing the conjugated antibodies in disease early diagnosis, which can provide a fast and promising clinical approach for virus differentiation and determination. In a word, a simple, fast, sensitive, and highly selective assay for EV71 and CVB3 has been developed. It could be applied in clinical sample analysis with a satisfactory result. It was notable that the sensor could not only achieve rapid and precise quantitative determination of protein/virus by fluorescent intensity but also could be applied in semiquantitative protein/virus determination by digital visualization.
引用
收藏
页码:3200 / 3207
页数:8
相关论文
共 37 条
[1]   Optimization of nanowire DNA sensor sensitivity using self-consistent simulation [J].
Baumgartner, S. ;
Vasicek, M. ;
Bulyha, A. ;
Heitzinger, C. .
NANOTECHNOLOGY, 2011, 22 (42)
[2]   Butterfly-Shaped Conjugated Oligoelectrolyte/Graphene Oxide Integrated Assay for Light-Up Visual Detection of Heparin [J].
Cai, Liping ;
Zhan, Ruoyu ;
Pu, Kan-Yi ;
Qi, Xiaoying ;
Zhang, Hua ;
Huang, Wei ;
Liu, Bin .
ANALYTICAL CHEMISTRY, 2011, 83 (20) :7849-7855
[3]   Transfer-Printing of Single DNA Molecule Arrays on Graphene for High-Resolution Electron Imaging and Analysis [J].
Cerf, Aline ;
Alava, Thomas ;
Barton, Robert A. ;
Craighead, Harold G. .
NANO LETTERS, 2011, 11 (10) :4232-4238
[4]   Highly selective adsorption of Hg2+ by a polypyrrole-reduced graphene oxide composite [J].
Chandra, Vimlesh ;
Kim, Kwang S. .
CHEMICAL COMMUNICATIONS, 2011, 47 (13) :3942-3944
[5]   Dual-Color Fluorescence and Homogeneous Immunoassay for the Determination of Human Enterovirus 71 [J].
Chen, Lu ;
Zhang, Xiaowei ;
Zhang, Cuiling ;
Zhou, Guohua ;
Zhang, Wanpo ;
Xiang, Dongshan ;
He, Zhike ;
Wang, Hanzhong .
ANALYTICAL CHEMISTRY, 2011, 83 (19) :7316-7322
[6]   Combining multiplex reverse transcription-PCR and a diagnostic microarray to detect and differentiate enterovirus 71 and coxsackievirus A16 [J].
Chen, Tsan-Chi ;
Chen, Guang-Wu ;
Hsiung, Chao Agnes ;
Yang, Jyh-Yuan ;
Shih, Shin-Ru ;
Lai, Yiu-Kay ;
Juang, Jyh-Lyh .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (06) :2212-2219
[7]   Nonvolatile Memory Device Using Gold Nanoparticles Covalently Bound to Reduced Graphene Oxide [J].
Cui, Peng ;
Seo, Sohyeon ;
Lee, Junghyun ;
Wang, Luyang ;
Lee, Eunkyo ;
Min, Misook ;
Lee, Hyoyoung .
ACS NANO, 2011, 5 (09) :6826-6833
[8]   Graphene Oxide as an Enzyme Inhibitor: Modulation of Activity of α-Chymotrypsin [J].
De, Mrinmoy ;
Chou, Stanley S. ;
Dravid, Vinayak P. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (44) :17524-17527
[9]   Electrochemical deposition of polypyrrole/graphene oxide composite on microelectrodes towards tuning the electrochemical properties of neural probes [J].
Deng, Meng ;
Yang, Xi ;
Silke, Musa ;
Qiu, Weiming ;
Xu, Mingsheng ;
Borghs, Gustaaf ;
Chen, Hongzheng .
SENSORS AND ACTUATORS B-CHEMICAL, 2011, 158 (01) :176-184
[10]   Fluorescence Resonance Energy Transfer between Quantum Dots and Graphene Oxide for Sensing Biomolecules [J].
Dong, Haifeng ;
Gao, Wenchao ;
Yan, Feng ;
Ji, Hanxu ;
Ju, Huangxian .
ANALYTICAL CHEMISTRY, 2010, 82 (13) :5511-5517