Morphological and molecular characterization and phylogenetic relationships of a new species of trypanosome in Tapirus terrestris (lowland tapir), Trypanosoma terrestris sp nov., from Atlantic Rainforest of southeastern Brazil

被引：11

作者：

Lima Acosta, Igor da Cunha ^{[1
]}

da Costa, Andrea Pereira ^{[1
]}

Nunes, Pablo Henrique ^{[2
]}

Naegeli Gondim, Maria Fernanda ^{[3
]}

Gatti, Andressa ^{[4
]}

Rossi, Joao Luiz, Jr. ^{[3
]}

Gennari, Solange Maria ^{[1
]}

Marcili, Arlei ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Vet Med, Dept Prevent Vet Med & Anim Sci, BR-05508 Sao Paulo, Brazil

[2] UNESP, Biosci Inst, Dept Biol, Rio Claro, SP, Brazil

[3] Univ Vila Velha, Fac Vet Med, Vila Velha, ES, Brazil

[4] Marcos Daniel Inst, Vitoria, ES, Spain

来源：

PARASITES & VECTORS | 2013年 / 6卷

基金：

巴西圣保罗研究基金会;

关键词：

Trypanosoma; Tapirs; Perissodactyla; Phylogeny; Atlantic rainforest; Taxonomy; SUBUNIT RIBOSOMAL-RNA; MEGATRYPANUM THEILERI; EVOLUTIONARY HISTORY; CYTOCHROME-B; SSU RDNA; PERISSODACTYLA; GENES; KINETOPLASTIDS; DEHYDROGENASE; RHINOCEROS;

D O I：

10.1186/1756-3305-6-349

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Background: The Lowland tapir (Tapirus terrestris) is the largest Brazilian mammal and despite being distributed in various Brazilian biomes, it is seriously endangered in the Atlantic Rainforest. These hosts were never evaluated for the presence of Trypanosoma parasites. Methods: The Lowland tapirs were captured in the Brazilian southeastern Atlantic Rainforest, Espirito Santo state. Trypanosomes were isolated by hemoculture, and the molecular phylogeny based on small subunit rDNA (SSU rDNA) and glycosomal-3-phosphate dehydrogenase (gGAPDH) gene sequences and the ultrastructural features seen via light microscopy and scanning and transmission electron microscopy are described. Results: Phylogenetic trees using combined SSU rDNA and gGAPDH data sets clustered the trypanosomes of Lowland tapirs, which were highly divergent from other trypanosome species. The phylogenetic position and morphological discontinuities, mainly in epimastigote culture forms, made it possible to classify the trypanosomes from Lowland tapirs as a separate species. Conclusions: The isolated trypanosomes from Tapirus terrestris are a new species, Trypanosoma terrestris sp. n., and were positioned in a new Trypanosoma clade, named T. terrestris clade.

引用

页数：12

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