Chitooligosaccharides suppress the level of protein expression and acetylcholinesterase activity induced by Aβ25-35 in PC12 cells

被引：31

作者：

Lee, Sang-Hoon ^{[1
]}

Park, Jin-Sook ^{[1
]}

Kim, Se-Kwon ^{[1
]}

Ahn, Chang-Bum ^{[2
]}

Je, Jae-Young ^{[2
]}

机构：

[1] Pukyong Natl Univ, Dept Chem, Pusan 608737, South Korea

[2] Chonnam Natl Univ, Div Food Sci & Aqualife Med, Yeosu 550749, South Korea

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2009年 / 19卷 / 03期

关键词：

Acetylcholinesterase; beta-Amyloid peptide; Chitooligosaccahride; Inhibition; ALZHEIMERS-DISEASE; BUTYRYLCHOLINESTERASE INHIBITORS; DERIVATIVES; HYPOTHESIS; SITE;

D O I：

10.1016/j.bmcl.2008.12.019

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Clinical applications of acetylcholinesterase (AChE) inhibitors are widespread in Alzheimer's sufferers in order to activate central cholinergic system and alleviate cognitive deficits by inhibiting the hydrolysis of acetylcholine. In this study, six kinds of chitooligosaccharides (COSs) with different molecular weight and degree of deacetylation were examined for their inhibitory effects against AChE. The 90-COSs exhibited potent AChE inhibitory activities compared to 50-COSs, while 90-MMWCOS (1000-5000 Da) in the 90-COSs showed the highest activity. Cell culture experiment revealed that 90-MMWCOS suppressed the level of AChE protein expression and AChE activity induced by A beta(25-35) in PC12 cell lines. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：860 / 862

页数：3

共 18 条

[1] Chemiluminescent high-throughput microassay applied to imidazo[2,1-b]thiazole derivatives as potential acetylcholinesterase and butyrylcholinesterase inhibitors [J].

Andreani, Aldo ;

Burnelli, Silvia ;

Granaiola, Massimiliano ;

Guardigli, Massimo ;

Leoni, Alberto ;

Locatelli, Alessandra ;

Morigi, Rita ;

Rambaldi, Mirella ;

Rizzoli, Manuela ;

Varoli, Lucilla ;

Roda, Aldo .

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2008, 43 (03) :657-661

[2] THE CHOLINERGIC HYPOTHESIS OF GERIATRIC MEMORY DYSFUNCTION [J].

BARTUS, RT ;

DEAN, RL ;

BEER, B ;

LIPPA, AS .

SCIENCE, 1982, 217 (4558) :408-417

[3] Toxic effects of acetylcholinesterase on neuronal and glial-like cells in vitro [J].

Calderon, FH ;

von Bernhardi, R ;

De Ferrari, G ;

Luza, S ;

Aldunate, R ;

Inestrosa, NC .

MOLECULAR PSYCHIATRY, 1998, 3 (03) :247-255

[4]

CHOI RCY, 2000, MOL NEUROSCI, V2, P801

[5]

Ellman L.G., 1961, BIOCHEM PHARMACOL, V2, P88

[6] SPECIAL PROPERTIES OF CHOLINESTERASES IN THE CEREBRAL-CORTEX OF ALZHEIMERS-DISEASE [J].

GEULA, C ;

MESULAM, M .

BRAIN RESEARCH, 1989, 498 (01) :185-189

[7] Cholinesterase inhibitors: new roles and therapeutic alternatives [J].

Giacobini, E .

PHARMACOLOGICAL RESEARCH, 2004, 50 (04) :433-440

[8] Medicine - The amyloid hypothesis of Alzheimer's disease: Progress and problems on the road to therapeutics [J].

Hardy, J ;

Selkoe, DJ .

SCIENCE, 2002, 297 (5580) :353-356

[9] Acetylcholinesterase accelerates assembly of amyloid-beta-peptides into Alzheimer's fibrils: Possible role of the peripheral site of the enzyme [J].

Inestrosa, NC ;

Alvarez, A ;

Perez, CA ;

Moreno, RD ;

Vicente, M ;

Linker, C ;

Casanueva, OI ;

Soto, C ;

Garrido, J .

NEURON, 1996, 16 (04) :881-891

[10] Free radical scavenging properties of hetero-chitooligosaccharides using an ESR spectroscopy [J].

Je, JY ;

Park, PJ ;

Kim, SK .

FOOD AND CHEMICAL TOXICOLOGY, 2004, 42 (03) :381-387

← 1 2 →