Functional expression and germline transmission of a human chromosome fragment in chimaeric mice

被引：209
作者：
Tomizuka, K
Yoshida, H
Uejima, H
Kugoh, H
Sato, K
Ohguma, A
Hayasaka, M
Hanaoka, K
Oshimura, M
Ishida, I
机构：
[1] KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN
[2] TOTTORI UNIV,FAC MED,SCH LIFE SCI,DEPT MOL & CELL GENET,YONAGO,TOTTORI 683,JAPAN
[3] KITASATO UNIV,SCH SCI,DEPT BIOSCI,MOL EMBRYOL LAB,SAGAMIHARA,KANAGAWA 228,JAPAN
来源：
NATURE GENETICS | 1997年 / 16卷 / 02期
关键词：
D O I：
10.1038/ng0697-133
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Human chromosomes or chromosome fragments derived from normal fibroblasts were introduced into mouse embryonic stem (ES) cells via microcell-mediated chromosome transfer (MMCT) and viable chimaeric mice were produced from them. Transferred chromosomes were stably retained, and human genes, including immunoglobulin (Ig) kappa, heavy, lambda genes, were expressed in proper tissue-specific manner in adult chimaeric tissues. In the case of a human chromosome (hChr.) 2-derived fragment, it was found to be transmitted to the offspring through the germline. Our study demonstrates that MMCT allows for introduction of very large amounts of foreign genetic material into mice. This novel procedure will facilitate the functional analyses of human genomes in vivo.
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页码：133 / 143
页数：11
相关论文
共 48 条
[1]   HUMAN-MOUSE HYBRIDS WITH AN EMBRYONAL CARCINOMA PHENOTYPE CONTINUE TO TRANSCRIBE HLA-A,B,C [J].
BENHAM, FJ ;
QUINTERO, MA ;
GOODFELLOW, PN .
EMBO JOURNAL, 1983, 2 (11) :1963-1968
[2]   THE CA-2+-BINDING PROTEIN PARVALBUMIN - MOLECULAR-CLONING AND DEVELOPMENTAL REGULATION OF MESSENGER-RNA ABUNDANCE [J].
BERCHTOLD, MW ;
MEANS, AR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (05) :1414-1418
[3]   Mammalian artificial chromosomes [J].
Brown, W ;
Heller, R ;
Loupart, ML ;
Shen, MH ;
Chand, A .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 1996, 6 (03) :281-288
[4]   A MAP OF THE HUMAN-IMMUNOGLOBULIN V-H LOCUS COMPLETED BY ANALYSIS OF THE TELOMERIC REGION OF CHROMOSOME 14Q [J].
COOK, GP ;
TOMLINSON, IM ;
WALTER, G ;
RIETHMAN, H ;
CARTER, NP ;
BULUWELA, L ;
WINTER, G ;
RABBITTS, TH .
NATURE GENETICS, 1994, 7 (02) :162-168
[5]  
DEVLIN BH, 1989, J BIOL CHEM, V264, P13896
[6]   MOSAIC MICE WITH TERATO-CARCINOMA-DERIVED MUTANT-CELLS DEFICIENT IN HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE [J].
DEWEY, MJ ;
MARTIN, DW ;
MARTIN, GR ;
MINTZ, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5564-5568
[7]   Efficient modification of human chromosomal alleles using recombination-proficient chicken human microcell hybrids [J].
Dieken, ES ;
Epner, EM ;
Fiering, S ;
Fournier, REK ;
Groudine, M .
NATURE GENETICS, 1996, 12 (02) :174-182
[8]   GENE TARGETING FOR SOMATIC-CELL MANIPULATION - RAPID ANALYSIS OF REDUCED CHROMOSOME HYBRIDS BY ALU-PCR FINGERPRINTING AND CHROMOSOME PAINTING [J].
DORIN, JR ;
EMSLIE, E ;
HANRATTY, D ;
FARRALL, M ;
GOSDEN, J ;
PORTEOUS, DJ .
HUMAN MOLECULAR GENETICS, 1992, 1 (01) :53-59
[9]   PRODUCTION OF VIABLE ADULT TRISOMY-17 [--] DIPLOID MOUSE CHIMERAS [J].
EPSTEIN, CJ ;
SMITH, SA ;
ZAMORA, T ;
SAWICKI, JA ;
MAGNUSON, TR ;
COX, DR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (14) :4376-4380
[10]   GENERATION OF A HUMAN X-DERIVED MINICHROMOSOME USING TELOMERE-ASSOCIATED CHROMOSOME FRAGMENTATION [J].
FARR, CJ ;
BAYNE, RAL ;
KIPLING, D ;
MILLS, W ;
CRITCHER, R ;
COOKE, HJ .
EMBO JOURNAL, 1995, 14 (21) :5444-5454
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