Fish protein hydrolysate in diets of turbot affects muscle fibre morphometry, and the expression of muscle growth-related genes

被引：13
作者：
Wei, Yuliang [1 ,2 ]
Li, Benxiang [1 ]
Xu, Houguo [1 ,2 ]
Liang, Mengqing [1 ,2 ]
机构：
[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, 1 Wenhai Rd, Qingdao 266237, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, 1 Wenhai Rd, Qingdao 266237, Peoples R China
来源：
AQUACULTURE NUTRITION | 2020年 / 26卷 / 05期
基金：
中国国家自然科学基金;
关键词：
fish protein hydrolysate; gene expression; growth performance; muscle fibre morphometry; muscle growth; turbot; MESSENGER-RNA EXPRESSION; SKELETAL-MUSCLE; PLANT PROTEIN; ATLANTIC SALMON; GRADED-LEVELS; PERFORMANCE; TEXTURE; SUPPLEMENTATION; DIGESTIBILITY; DIFFERENTIATION;
D O I：
10.1111/anu.13129
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The study was conducted to investigate the effects of fish protein hydrolysate (FPH) in diets for turbot on growth performance, muscle fibre morphometry, and the expression of muscle growth-related genes. A control diet (FPH0) contained 0 g/kg FPH, and four experimental diets were formulated replacing fishmeal with FPH at levels of 45 (FPH4.5), 120 (FPH12), 180 (FPH18) and 300 (FPH30) g/kg. Fish fed the FPH12 and FPH18 diets had higher specific growth rate (SGR) than fish fed the FPH0 diet (p < .05), and a quadratic regression was found between SGR and dietary FPH level (p = .001,R-2 = .677). Cross-section area (CSA) and the length of sarcomere in the FPH12 group increased compared with the control group (p < .05), and a quadratic regression was observed between CSA and dietary FPH level (p = .006,R-2 = .574) and between sarcomere length and dietary FPH level (p = .018,R-2 = .788). An appropriate level of FPH down-regulated myostatin 2 gene expression and up-regulated proliferating cell nuclear antigen gene expression, while the expression of myogenic regulatory factors was not affected by dietary treatments (p > .05). To conclude, an appropriate level of FPH may improve muscle growth by regulating the expression of muscle growth-related genes, and muscle microstructure and ultrastructure.
引用
收藏
页码：1780 / 1791
页数：12
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