Differential display analysis of gene expression accompanied by neurite outgrowth of human neuroblastoma Cell IMR32 using non-gel molecular sieving capillary electrophoresis

被引：0
作者：
Ishioka, N
Kurosu, Y
Kuhara, A
Kogure, T
Ueno, Y
Saito, M
Watabe, K
Nagaoka, I
机构：
[1] Natl Space Dev Agcy Japan, Tsukuba Space Ctr, Tsukuba, Ibaraki 3058505, Japan
[2] Juntendo Univ, Sch Med, Dept Biochem, Bunkyo Ku, Tokyo 1130033, Japan
[3] JASCO Tech Res Labs Corp, Hachioji, Tokyo 1920032, Japan
[4] Jikei Univ, Sch Med, Inst DNA Med, Div Mol Cell Biol,Minato Ku, Tokyo 1050003, Japan
[5] Jikei Univ, Sch Med, Dept Neurosurg, Minato Ku, Tokyo 1050003, Japan
[6] Jikei Univ, Sch Med, Dept Orthoped Surg, Minato Ku, Tokyo 1050003, Japan
[7] Tokyo Metropolitan Inst Neurosci, Dept Neuropathol, Tokyo 1830042, Japan
来源：
ANALUSIS | 1999年 / 27卷 / 02期
关键词：
differential display; RT-PCR; neuroblastoma; cell proliferation; capillary electrophoresis; molecular sieving; linear polyacrylamide;
D O I：
10.1051/analusis:1999163
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The induced proliferative response of the human derived neuroblastoma cell (IMR32), with the cell proliferation mediating factor, is much milder compared to other cell strains, and develops cellular clusters which are characterized by numerous extensions of dendrites. Presuming this phenomenon to be one of the induced neuronal differentiation due to genetic alterations, we preliminary studied to elucidate the changes in gene expression of the IMR32 by mRNA differential display analysis, monitoring polymerase chain reaction (PCR) products by non-gel molecular sieving capillary electrophoresis in linear polyacrylamide solution. The CE fingerprints revealed a number of peaks with differential expression patterns.
引用
收藏
页码：166 / 169
页数：4
相关论文
共 20 条
  • [1] Automated polymerase chain reaction product sample preparation for capillary electrophoresis analysis
    Belgrader, P
    Devaney, JM
    DelRio, SA
    Turner, KA
    Weaver, KR
    Marino, MA
    [J]. JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 683 (01): : 109 - 114
  • [2] ANALYTICAL AND MICROPREPARATIVE CAPILLARY ELECTROPHORESIS OF THE PEPTIDES FROM CALCITONIN
    CAMILLERI, P
    OKAFO, GN
    SOUTHAN, C
    BROWN, R
    [J]. ANALYTICAL BIOCHEMISTRY, 1991, 198 (01) : 36 - 42
  • [3] Detection of a genetic variant, lysine->glutamic acid at position 372 of human serum albumin, by capillary electrophoresis and structural identification
    Ishioka, N
    Kogure, T
    Kurosu, Y
    [J]. JOURNAL OF CHROMATOGRAPHY B, 1997, 697 (1-2): : 135 - 140
  • [4] FLUORESCENT DIFFERENTIAL DISPLAY - ARBITRARILY PRIMED RT-PCR FINGERPRINTING ON AN AUTOMATED DNA SEQUENCER
    ITO, T
    KITO, K
    ADATI, N
    MITSUI, Y
    HAGIWARA, H
    SAKAKI, Y
    [J]. FEBS LETTERS, 1994, 351 (02) : 231 - 236
  • [5] KRSTULOVIC AM, 1997, ELECTROPHORESIS LIFE
  • [6] DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER-RNA BY MEANS OF THE POLYMERASE CHAIN-REACTION
    LIANG, P
    PARDEE, AB
    [J]. SCIENCE, 1992, 257 (5072) : 967 - 971
  • [7] LIANG P, 1992, CANCER RES, V52, P6966
  • [8] DISTRIBUTION AND CLONING OF EUKARYOTIC MESSENGER-RNAS BY MEANS OF DIFFERENTIAL DISPLAY - REFINEMENTS AND OPTIMIZATION
    LIANG, P
    AVERBOUKH, L
    PARDEE, AB
    [J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (14) : 3269 - 3275
  • [9] Identifying changes in gene expression in the nervous system: mRNA differential display
    Livesey, FJ
    Hunt, SP
    [J]. TRENDS IN NEUROSCIENCES, 1996, 19 (03) : 84 - 88
  • [10] CAPILLARY ELECTROPHORESIS OF POLYMERASE CHAIN REACTION-AMPLIFIED DNA USING FLUORESCENCE DETECTION WITH AN INTERCALATING DYE
    MCCORD, BR
    MCCLURE, DL
    JUNG, JM
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1993, 652 (01) : 75 - 82
12