Low Molecular Weight Chitosan Accelerates Glucagon-like Peptide-1 Secretion in Human Intestinal Endocrine Cells via a p38-Dependent Pathway

被引:9
作者
Liu, Shing Hwa [1 ]
Huang, Ya Wen [2 ]
Wu, Cheng Tien [1 ]
Chiu, Chen Yuan [1 ]
Chiang, Meng Tsan [2 ]
机构
[1] Natl Taiwan Univ, Inst Toxicol, Coll Med, Taipei 10764, Taiwan
[2] Natl Taiwan Ocean Univ, Coll Life Sci, Dept Food Sci, Keelung, Taiwan
关键词
chitosan; glucagon-like peptide-1; proglucagon; p38; MAPK; intestine; PROGLUCAGON GENE-EXPRESSION; PROHORMONE CONVERTASES; INSULIN-RESISTANCE; LONG-TERM; GLUCOSE; CHITOOLIGOSACCHARIDES; OLIGOSACCHARIDES; ABSORPTION; OVERWEIGHT; CHITIN;
D O I
10.1021/jf305410k
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Chitosan is widely employed as a dietary supplement. Several studies have shown that chitosan possesses an antidiabetic effect. An important intestinal incretin hormone, glucagon-like peptide-1 (GLP-1), is also known to contribute to the amelioration of diabetes. This study investigated whether chitosan possesses an ability in GLP-1 synthesis and secretion in human intestinal cells. Low molecular weight chitosan (LMWC) significantly increases GLP-1 secretion in human intestinal endocrine cells (NCI-H716) in a dose-dependent manner. LMWC could also dose-dependently increase the mRNA expression of proglucagon, a GLP-1 precursor, but did not affect prohormone convertase 3 (PC 3) mRNA expression. LMWC effectively increased die phosphorylation of mitogen-activated protein kinases (MAPK)-p38 and c-Jun N-terminal kinases (JNK), but not extracellular-signal-regulated kinases (ERK). An inhibitor of p38, but not JNK and ERK, significantly reversed the LMWC-increased proglucagon expression. Taken together, LMWC accelerates proglucagon expression and GLP-1 secretion through a p38/MAPK-dependent signaling pathway. These findings suggest that LMWC may provide a strategy for diabetes therapy.
引用
收藏
页码:4855 / 4861
页数:7
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