Comparative tests on the efficiency of three methods of methylmercury extraction in environmental samples

被引:1
作者
Brito, EMS [1 ]
Guimaraes, JRD [1 ]
机构
[1] UFRJ Ilha do Fundao, Inst Biofis Carlos Chagas, Lab Radioisotopos Eduardo Penna Franca, BR-21949900 Rio De Janeiro, Brazil
关键词
methylmercury; freshwater systems; sediment; macrophytes; extraction;
D O I
10.1002/(SICI)1099-0739(199907)13:7<487::AID-AOC862>3.0.CO;2-B
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Mercury is used in gold mining in the Brazilian Amazon and released in significant amounts to the environment as Hg-0 After its oxidation it may be methylated, mainly in bottom sediments and in the rhizosphere of floating aquatic macrophytes. Methylmercury (MeHg) is highly bioavailable and subject to biomagnification. The objective of this work was to evaluate the efficiency of three methylmercury extraction techniques, applied to replicates of environmental samples (stream sediments from Floresta da Tijuca, Rio de Janeiro; and roots of the floating macrophyte Eichhornia crassipes from Lagoinha, Rio de Janeiro) previously incubated with Hg-203(2+). Method A is based on acid leaching and extraction of (MeHg)-Hg-203 in toluene. Method B uses alkaline digestion, extraction in dithizone-benzene and separation of organic and inorganic Hg-203 dithizonates by thin-layer chromatography (TLC). Method C consists in separating the (MeHg)-Hg-203 from the sample matrix by distillation. Total Hg-203 and (MeHg)-Hg-203 were detected by gamma spectrometry or liquid scintillation. For both matrices, (MeHg)-Hg-203 extraction efficiency was better for smaller samples (range: 0.08-0.5 g for sediment, 0.1-0.5 g for E. crassipes roots). For the sediment samples, the three selected methods presented similar (MeHg)-Hg-203 extraction efficiencies: 7-13, 5-14 and 4-17% of total added Hg-203 was found as MeHg for procedures A, B and C, respectively. For E. crassipes roots, on the other hand, a lower extraction efficiency was obtained for the procedure C (11-28% of total Hg-203 present as (MeHg)-Hg-203) than for methods B (22-36%) and A (20-44%). In all the root samples prepared with procedure B, a strong and durable chemiluminescence effect was observed, which required measuring the final (MeHg)-Hg-203 extracts by gamma spectrometry only. In the specific conditions we used, extraction via distillation required reextraction of (MeHg)-Hg-203 in the distillate, due to the presence of traces of inorganic Hg-203 in the latter. Copyright (C) 1999 John Wiley & Sons, Ltd.
引用
收藏
页码:487 / 493
页数:7
相关论文
共 50 条
  • [21] Comparative Evaluation of Three Different Extraction Methods for Rice (Oryza sativa L.) Genomic DNA
    Sagi, Naoki
    Monma, Kimio
    Ibe, Akihiro
    Kamata, Kunihiro
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (07) : 2745 - 2753
  • [22] Methylmercury determination in biological samples using electrothermal atomic absorption spectrometry after acid leaching extraction
    Mohammad Saber-Tehrani
    Hamid Hashemi-Moghaddam
    Mohammad Hadi Givianrad
    Parviz Abroomand-Azar
    [J]. Analytical and Bioanalytical Chemistry, 2006, 386 : 1407 - 1412
  • [23] Rapid extraction and assay of uranium from environmental surface samples
    Barrett, Christopher A.
    Chouyyok, Wilaiwan
    Speakman, Robert J.
    Olsen, Khris B.
    Addleman, Raymond Shane
    [J]. TALANTA, 2017, 173 : 69 - 78
  • [24] EXTRACTION-SPECTROPHOTOMETRIC DETERMINATION OF TIN IN ENVIRONMENTAL-SAMPLES
    CHAKRABORTY, AR
    PATEL, KS
    MISHRA, RK
    [J]. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, 1991, 45 (04) : 229 - 233
  • [25] Arsenic speciation in environmental and biological samples -: Extraction and stability studies
    Pizarro, I
    Gómez, M
    Cámara, C
    Palacios, MA
    [J]. ANALYTICA CHIMICA ACTA, 2003, 495 (1-2) : 85 - 98
  • [26] Methylmercury determination in biological samples using electrothermal atomic absorption spectrometry after acid leaching extraction
    Saber-Tehrani, Mohammad
    Hashemi-Moghaddam, Hamid
    Givianrad, Mohammad Hadi
    Abroomand-Azar, Parviz
    [J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 386 (05) : 1407 - 1412
  • [27] Methods and recent advances in speciation analysis of mercury chemical species in environmental samples: a review
    Amde, Meseret
    Yin, Yongguang
    Zhang, Dan
    Liu, Jingfu
    [J]. CHEMICAL SPECIATION AND BIOAVAILABILITY, 2016, 28 (1-4): : 51 - 65
  • [28] Comparative analysis of environmental DNA extraction and purification methods from different humic acid-rich soils
    Lakay, F. M.
    Botha, A.
    Prior, B. A.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2007, 102 (01) : 265 - 273
  • [29] Determination of methylmercury in biological samples using dithizone extraction method followed by purge & trap GC-MS
    Lee, Jung-Sub
    Ryu, Yoon-Jung
    Park, Jae-Sung
    Jeon, Sung-Hwan
    Kim, Sam-Cwan
    Kim, Young-Hee
    [J]. BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2007, 28 (12) : 2293 - 2298
  • [30] Supercritical fluid carbon dioxide extraction and liquid chromatographic separation with electrochemical detection of methylmercury from biological samples
    Simon, NS
    [J]. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, 1997, 68 (03) : 313 - 330