Small-volume vitrification for human spermatozoa in the absence of cryoprotectants by using Cryotop

被引：39
作者：
Chen, Y. [1 ,2 ]
Li, L. [2 ]
Qian, Y. [2 ]
Xu, C. [2 ]
Zhu, Y. [2 ]
Huang, H. [2 ]
Jin, F. [2 ]
Ye, Y. [2 ]
机构：
[1] Hubei Univ Med, Taihe Hosp, Reprod Med Ctr, Shiyan, Hubei, Peoples R China
[2] Zhejiang Univ, Sch Med, Womens Hosp, Dept Reprod Endocrinol, Hangzhou 310006, Zhejiang, Peoples R China
来源：
ANDROLOGIA | 2015年 / 47卷 / 06期
关键词：
Cryoprotective agents; Cryotop; spermatozoa; sucrose; vitrification; DNA FRAGMENTATION; SMALL NUMBERS; HUMAN SPERM; CRYOPRESERVATION; TECHNOLOGY; INTEGRITY; MOTILITY; ABILITY;
D O I：
10.1111/and.12320
中图分类号：
R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
摘要：
Cryotop is a carrier that has been used successfully in the cryopreservation of human spermatozoa. Here, we explored a novel method to vitrify human spermatozoa without cryoprotective agents (CPAs) using Cryotop. Spermatozoa from 21 Normozoospermic patients were collected and vitrified without CPAs or with sucrose in small volume using Cryotop. The sperm recovery rate, motility, viability, chromatin damage and DNA fragmentation were assessed. No significant difference was observed in the sperm recovery rate and motility rate between the spermatozoa cryopreserved without CPAs and with sucrose. The post-thawed spermatozoa cryopreserved without CPAs had a higher viability and lower damage to sperm chromatin and DNA than those cryopreserved with sucrose. These results suggest that small numbers of human spermatozoa can be successfully vitrified without CPAs using Cryotop.
引用
收藏
页码：694 / 699
页数：6
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