Evaluation of Anti-Mycobacterial Compounds in a Silkworm Infection Model with Mycobacteroides abscessus

被引：18

作者：

Hosoda, Kanji ^{[1
,2
]}

Koyama, Nobuhiro ^{[1
,2
]}

Hamamoto, Hiroshi ^{[3
]}

Yagi, Akiho ^{[4
]}

Uchida, Ryuji ^{[4
]}

Kanamoto, Akihiko ^{[5
]}

Tomoda, Hiroshi ^{[1
,2
]}

机构：

[1] Kitasato Univ, Grad Sch Pharmaceut Sci, Dept Microbial Chem, Tokyo 1088641, Japan

[2] Kitasato Univ, Sch Pharm, Med Res Labs, Tokyo 1088641, Japan

[3] Teikyo Univ, Inst Med Mycol, Tokyo 1920395, Japan

[4] Tohoku Med & Pharmaceut Univ, Fac Pharmaceut Sci, Sendai, Miyagi 9818558, Japan

[5] OP Bio Factory Co Ltd, Uruma, Okinawa 9042234, Japan

来源：

MOLECULES | 2020年 / 25卷 / 21期

关键词：

silkworm; mycobacteria; nontuberculous mycobacteria; Mycobacterium avium complex; Mycobacteroides abscessus; antimycobacterial activity; microbial product; lariatin; nosiheptide; ohmyungsamycin; steffimycin; quinomycin; QUANTITATIVE-EVALUATION; ANTIBIOTICS; PEPTIDES; DISEASE; NOSOKOMYCINS; QUINOMYCINS;

D O I：

10.3390/molecules25214971

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Among four mycobacteria, Mycobacterium avium, M. intracellulare, M. bovis BCG and Mycobacteroides (My.) abscessus, we established a silkworm infection assay with My. abscessus. When silkworms (fifth-instar larvae, n = 5) were infected through the hemolymph with My. abscessus (7.5 x 10(7) CFU/larva) and bred at 37 degrees C, they all died around 40 h after injection. Under the conditions, clarithromycin and amikacin, clinically used antimicrobial agents, exhibited therapeutic effects in a dose-dependent manner. Furthermore, five kinds of microbial compounds, lariatin A, nosiheptide, ohmyungsamycins A and B, quinomycin and steffimycin, screened in an in vitro assay to observe anti-My. abscessus activity from 400 microbial products were evaluated in this silkworm infection assay. Lariatin A and nosiheptide exhibited therapeutic efficacy. The silkworm infection model with My. abscessus is useful to screen for therapeutically effective anti-My. abscessus antibiotics.

引用

页数：11