Imaging through scattering microfluidic channels by digital holography for information recovery in lab on chip

被引：39

作者：

Bianco, V. ^{[1
]}

Paturzo, M. ^{[1
]}

Gennari, O. ^{[1
]}

Finizio, A. ^{[1
]}

Ferraro, P. ^{[1
]}

机构：

[1] CNR, Ist Nazl Ott, Sez Napoli, I-80078 Naples, Italy

来源：

OPTICS EXPRESS | 2013年 / 21卷 / 20期

关键词：

BACTERIAL BIOFILM; SPATIAL COHERENCE; ON-CHIP; MICROSCOPY; DEVICES; RECONSTRUCTION; TRANSPORT; REDUCTION; DYNAMICS; SAMPLES;

D O I：

10.1364/OE.21.023985

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We tackle the problem of information recovery and imaging through scattering microfluidic chips by means of digital holography (DH). In many cases the chip can become opalescent due to residual deposits settling down the inner channel faces, biofilm formation, scattering particle uptake by the channel cladding or its damaging by corrosive substances, or even by condensing effect on the exterior channels walls. In these cases white-light imaging is severely degraded and no information is obtainable at all about the flowing samples. Here we investigate the problem of counting and estimating velocity of cells flowing inside a scattering chip. Moreover we propose and test a method based on the recording of multiple digital holograms to retrieve improved phase-contrast images despite the strong scattering effect. This method helps, thanks to DH, to recover information which, otherwise, would be completely lost. (C)2013 Optical Society of America

引用

页码：23985 / 23996

页数：12

共 57 条

[1] Nondestructive testing by using long-wave infrared interferometric techniques with CO2 lasers and microbolometer arrays [J].