19-VNTR loci used in genotyping Chinese clinical Mycobacterium tuberculosis complex strains and in association with spoligotyping

被引:6
作者
Jiang, Yi [1 ]
Liu, Hai-can [1 ]
Zheng, Huajun [2 ]
Dou, Xiangfeng [3 ]
Tang, Biao [2 ]
Zhao, Xiu-qin [1 ]
Zhu, Yongqiang [2 ]
Lu, Bing [1 ]
Wang, Shengyue [2 ]
Dong, Hai-yan [1 ]
Zhang, Yuan-yuan [1 ]
Zhao, Guoping [2 ]
Wan, Kanglin [1 ]
机构
[1] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China
[2] Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai, Peoples R China
[3] Beijing Ctr Dis Prevent & Control, Beijing, Peoples R China
关键词
Mycobacterium tuberculosi; VNTR; VARIABLE-NUMBER; TANDEM REPEATS; BEIJING FAMILY; REPETITIVE UNITS; DRUG-RESISTANT; DIFFERENTIATION; UTILITY; IDENTIFICATION; COMBINATION; DIVERSITY;
D O I
10.1002/jobm.201200230
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Recently, tandem repeat typing has emerged as a rapid and easy method for the molecular epidemiology of the Mycobacterium tuberculosis (M. tuberculosis) complex. In this study, a collection of 19 VNTRs incorporating 15 previously described loci and 4 newly evaluated markers were used to genotype 206 Chinese M. tuberculosis isolates and 9 BCG strains. The discriminatory power was evaluated and compared with that obtained by Spoligotyping. It turned out that 15-locus VNTR could be very useful in M. tuberculosis complex strains genotyping in China. The 4 newly evaluated loci were proved informative and could be useful for future epidemiology studies, especially in Beijing family strains. In addition, a unique pattern of the latter 4 loci were found in Chinese BCG strains.
引用
收藏
页码:562 / 580
页数:19
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