Novel biosensor platforms for the detection of coronavirus infection and severe acute respiratory syndrome coronavirus 2

被引：17

作者：

Liang, Kung-Hao ^{[1
,2
,3
]}

Chang, Tai-Jay ^{[4
,5
]}

Wang, Mong-Lien ^{[2
,6
]}

Tsai, Ping-Hsing ^{[7
]}

Lin, Ta-Hsien ^{[3
,8
]}

Wang, Chin-Tien ^{[9
,10
]}

Yang, De-Ming ^{[11
,12
,13
]}

机构：

[1] Taipei Vet Gen Hosp, Dept Med Res, Lab Syst Biomed Sci, Taipei, Taiwan

[2] Natl Yang Ming Univ, Inst Food Safety & Hlth Risk Assessment, Taipei, Taiwan

[3] Natl Yang Ming Univ, Sch Med, Inst Biomed Informat, Taipei, Taiwan

[4] Taipei Vet Gen Hosp, Basic Res Div, Dept Med Res, Lab Genome Res, Taipei, Taiwan

[5] Natl Yang Ming Univ, Sch Biomed Sci & Engn, Taipei, Taiwan

[6] Taipei Vet Gen Hosp, Dept Med Res, Lab Mol Oncol, Taipei, Taiwan

[7] Taipei Vet Gen Hosp, Div Basic Res, Dept Med Res, Lab Stem Cell Res 2, Taipei, Taiwan

[8] Taipei Vet Gen Hosp, Dept Med Res, Basic Res Div, Lab Nucl Magnet Resonance, Taipei, Taiwan

[9] Taipei Vet Gen Hosp, Lab Mol Virol, Basic Res Div, Dept Med Res, Taipei, Taiwan

[10] Natl Yang Ming Univ, Sch Med, Inst Clin Med, Taipei, Taiwan

[11] Taipei Vet Gen Hosp, Dept Med Res, Basic Res Div, Microscopy Serv Lab, 201,Sect 2,Shi Pai Rd, Taipei 112, Taiwan

[12] Natl Yang Ming Univ, Sch Biomed Sci & Engn, Inst Biophoton, Taipei, Taiwan

[13] Natl Yang Ming Univ, Biophoton & Mol Imaging Res Ctr BMIRC, Taipei, Taiwan

来源：

JOURNAL OF THE CHINESE MEDICAL ASSOCIATION | 2020年 / 83卷 / 08期

关键词：

Aerosol; Coronavirus disease 2019; Fluorescence resonance energy transfer-based biosensors; Protein-protein interactions; Real-time reverse transcription polymerase chain reaction; qPCR; Severe acute respiratory syndrome coronavirus 2; 2019-nCov; SARS-ASSOCIATED CORONAVIRUS; GREEN FLUORESCENT PROTEINS; NUCLEOCAPSID PROTEIN; SPIKE PROTEIN; IDENTIFICATION; INDICATORS; ANTIBODIES; CA2+;

D O I：

10.1097/JCMA.0000000000000337

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The recent outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has been causing respiratory diseases globally, damaging wide ranges of social-economic activities. This virus is transmitted through personal contact and possibly also through ambient air. Effective biosensor platforms for the detection of this virus and the related host response are in urgent demand. These platforms can facilitate routine diagnostic assays in certified clinical laboratories. They can also be integrated into point-of-care products. Furthermore, environmental biosensors can be designed to detect SARS-CoV-2 in the ambient air or in the intensive care ventilators. Here, we evaluate technical components of biosensors, including the biological targets of recognition, the recognition methods, and the signal amplification and transduction systems. Effective SARS-CoV-2 detectors can be designed by an adequate combination of these technologies.

引用

页码：701 / 703

页数：3

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