Enzymatic synthesis of a series of alkyl esters using novozyme 435 in a packed-bed, miniaturized, continuous flow reactor

被引：37

作者：

Woodcock, L. L. ^{[1
]}

Wiles, C. ^{[1
]}

Greenway, G. M. ^{[1
]}

Watts, P. ^{[1
]}

Wells, A. ^{[2
]}

Eyley, S. ^{[2
]}

机构：

[1] Univ Hull, Kingston Upon Hull HU6 7RX, East Yorkshire, England

[2] AstraZeneca UK Ltd, Loughborough, Leics, England

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2008年 / 26卷 / 06期

基金：

英国工程与自然科学研究理事会;

关键词：

Enzymatic synthesis; alkyl esters; novozyme; 435; miniaturized; flow reactor;

D O I：

10.1080/10242420802456571

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using lipase catalysed, enzymatic esterification as a model reaction, we successfully demonstrate the use of miniaturized technology for biocatalytic reactions. Benchmarked against batch reactions, nine alkyl esters have been synthesized effectively, using Novozyme 435 in a pressure driven, packed-bed, miniaturized, continuous flow reactor. In some cases close to 100% ester conversions were obtained. The paper also demonstrates the ability to screen the enzyme for substrate specificity.

引用

页码：501 / 507

页数：7

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