Long PCR-amplified rDNA for PCR-RFLP- and Rep-PCR-based approaches to recognize closely related microbial species

被引：16
作者：
Abd-El-Haleem, D
Layton, AC
Sayler, GS [1 ]
机构：
[1] Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37996 USA
[2] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA
[3] Mubarak City Sci Res & Technol Applicat, GEBRI, Environm Biotechnol Dept, Alexandria, Egypt
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2002年 / 49卷 / 03期
关键词：
ribosomal operon; Ralstonia; long PCR;
D O I：
10.1016/S0167-7012(01)00374-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Long PCR was used to amplify a 5-kb fragment of the bacterial ribosomal operon (16S-intergenic spacer region (ISR)-23S) from several Ralstonia eutropha strains (16S rDNA sequence similarity: 97-99%). Due to the large product size, amplicons from the different strains could be distinguished using restriction enzyme fragment length polymorphisms (RFLP) and repetitive PCR analysis (Rep-PCR) with the primer 1492r. These methods may prove useful in differentiating other bacterial strains with highly similar 16S rDNA sequences. (C) 2002 Elsevier Science B.V. All rights reserved.
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收藏
页码：315 / 319
页数：5
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