Separation and quantification of nine bioactive compounds in traditional Unani formulations by High Performance Liquid Chromatography-Photodiode Array Detector

被引：6

作者：

Kamal, Y. T. ^{[1
]}

Ahmad, Sayeed ^{[2
]}

Mahadevan, Nanjaian ^{[1
]}

Alam, Prawez ^{[3
]}

Salam, Shahana ^{[4
]}

Asiri, Yahya, I ^{[5
]}

Bin Muhsinah, Abdullatif ^{[1
]}

Alsayari, Abdulrhman ^{[1
]}

机构：

[1] King Khalid Univ, Coll Pharm, Dept Pharmacognosy, Abha 61441, Saudi Arabia

[2] Jamia Hamdard, Dept Pharmacognosy & Phytochem, Bioact Nat Prod Lab, Fac Pharm, New Delhi, India

[3] Prince Sattam Bin Abdulaziz Univ, Coll Pharm, Dept Pharmacognosy, POB 173, Al Kharj 11942, Saudi Arabia

[4] Prince Sattam Bin Abdulaziz Univ, Dept Pharmaceut Chem, Coll Pharm, POB 173, Al Kharj 11942, Saudi Arabia

[5] King Khalid Univ, Coll Pharm, Dept Pharmacol, Abha 61441, Saudi Arabia

来源：

ACTA CHROMATOGRAPHICA | 2021年 / 33卷 / 01期

关键词：

HPLC-PDA; quality control; polyherbal formulation; simultaneous determination; PHENOLIC-COMPOUNDS; HPLC ANALYSIS; BULK DRUG; RP-HPLC; EXTRACTS;

D O I：

10.1556/1326.2020.00696

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new High Performance Liquid Chromatography-Photodiode Array Detector (HPLC-PDA) method has been developed for the chromatographic separation and simultaneous quantitative determination of nine bioactive compounds, i.e. four phenolic (gallic acid, ellagic acid, chebulinic acid, and tannic acid), two flavanoids (rutin and quercetin), two anthraquinones (sennoside A and B) and one oxygenated hydrocarbon (vitamin C) in a well-known Unani polyherbal formulation namely Itrifal's. Separation was accomplished on a C18 LiChrospher 100 column (5 mm, 250 x 4.6 mm) with a gradient elution and recorded at 254 nm. The results demonstrated that the proposed method is reproducible, accurate, economic, and suitable for the quality control of traditional polyherbal Unani formulations containing complex compounds with different structures such as Itrifals.

引用

页码：3 / 10

页数：8

共 20 条

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