Characterization of Pro-Inflammatory Flagellin Proteins Produced by Lactobacillus ruminis and Related Motile Lactobacilli

被引：63

作者：

Neville, B. Anne ^{[1
]}

Forde, Brian M. ^{[1
]}

Claesson, Marcus J. ^{[1
]}

Darby, Trevor ^{[2
]}

Coghlan, Avril ^{[1
]}

Nally, Kenneth ^{[2
]}

Ross, R. Paul ^{[3
]}

O'Toole, Paul W. ^{[1
,2
]}

机构：

[1] Natl Univ Ireland Univ Coll Cork, Dept Microbiol, Cork, Ireland

[2] Natl Univ Ireland Univ Coll Cork, Alimentary Pharmabiot Ctr, Cork, Ireland

[3] TEAGASC, Moorepk Food Res Ctr, Fermoy, Cork, Ireland

来源：

PLOS ONE | 2012年 / 7卷 / 07期

基金：

爱尔兰科学基金会;

关键词：

LACTIC-ACID BACTERIA; INTESTINAL EPITHELIAL-CELLS; INNATE IMMUNE-RESPONSE; ESCHERICHIA-COLI; GENOME SEQUENCES; GENE-EXPRESSION; RHAMNOSUS GG; SP NOV; STRAINS; SURFACE;

D O I：

10.1371/journal.pone.0040592

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Lactobacillus ruminis is one of at least twelve motile but poorly characterized species found in the genus Lactobacillus. Of these, only L. ruminis has been isolated from mammals, and this species may be considered as an autochthonous member of the gastrointestinal microbiota of humans, pigs and cows. Nine L. ruminis strains were investigated here to elucidate the biochemistry and genetics of Lactobacillus motility. Six strains isolated from humans were non-motile while three bovine isolates were motile. A complete set of flagellum biogenesis genes was annotated in the sequenced genomes of two strains, ATCC25644 (human isolate) and ATCC27782 (bovine isolate), but only the latter strain produced flagella. Comparison of the L. ruminis and L. mali DSM20444(T) motility loci showed that their genetic content and gene-order were broadly similar, although the L. mali motility locus was interrupted by an 11.8 Kb region encoding rhamnose utilization genes that is absent from the L. ruminis motility locus. Phylogenetic analysis of 39 motile bacteria indicated that Lactobacillus motility genes were most closely related to those of motile carnobacteria and enterococci. Transcriptome analysis revealed that motility genes were transcribed at a significantly higher level in motile L. ruminis ATCC27782 than in non-motile ATCC25644. Flagellin proteins were isolated from L. ruminis ATCC27782 and from three other Lactobacillus species, while recombinant flagellin of aflagellate L. ruminis ATCC25644 was expressed and purified from E. coli. These native and recombinant Lactobacillus flagellins, and also flagellate L. ruminis cells, triggered interleukin-8 production in cultured human intestinal epithelial cells in a manner suppressed by short interfering RNA directed against Toll-Like Receptor 5. This study provides genetic, transcriptomic, phylogenetic and immunological insights into the trait of flagellum-mediated motility in the lactobacilli.

引用

页数：12

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