PROCESS PARAMETERS STUDY OF α-AMYLASE PRODUCTION IN A PACKED-BED BIOREACTOR UNDER SOLID-STATE FERMENTATION WITH POSSIBILITY OF TEMPERATURE MONITORING

被引：10
作者：
Derakhti, Sorour [1 ]
Shojaosadati, Seyed Abbas [1 ]
Hashemi, Maryam [2 ]
Khajeh, Khosro [3 ]
机构：
[1] Tarbiat Modares Univ, Dept Chem Engn, Biotechnol Grp, Tehran, Iran
[2] ABRII, Dept Microbial Biotechnol & Biosafety, Karaj, Iran
[3] Tarbiat Modares Univ, Fac Sci, Dept Biochem & Biophys, Tehran, Iran
来源：
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2012年 / 42卷 / 03期
关键词：
alpha-amylase; Bacillus sp KR-8104; packed-bed bioreactor; response surface methodology; solid-state fermentation; BACILLUS-SUBTILIS; OPTIMIZATION;
D O I：
10.1080/10826068.2011.599466
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Production of alpha-amylase in a laboratory-scale packed-bed bioreactor by Bacillus sp. KR-8104 under solid-state fermentation (SSF) with possibility of temperature control and monitoring was studied using wheat bran (WB) as a solid substrate. The simultaneous effects of aeration rate, initial substrate moisture, and incubation temperature on alpha-amylase production were evaluated using response surface methodology (RSM) based on a Box-Behnken design. The optimum conditions for attaining the maximum production of alpha-amylase were 37 degrees C, 72% (w/w) initial substrate moisture, and 0.15 L/min aeration. The average enzyme activity obtained under the optimized conditions was 473.8 U/g dry fermented substrate. In addition, it was observed that the production of enzyme decreased from the bottom of the bioreactor to the top.
引用
收藏
页码：203 / 216
页数：14
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